Comammox Nitrospira are abundant ammonia oxidizers in diverse groundwater-fed rapid sand filter communities

Environmental Microbiology
Susan Jane FowlerBarth F Smets

Abstract

The recent discovery of completely nitrifying Nitrospira demands a re-examination of nitrifying environments to evaluate their contribution to nitrogen cycling. To approach this challenge, tools are needed to detect and quantify comammox Nitrospira. We present primers for the simultaneous quantification and diversity assessement of both comammox Nitrospira clades. The primers cover a wide range of comammox diversity, spanning all available high quality sequences. We applied these primers to 12 groundwater-fed rapid sand filters, and found comammox Nitrospira to be abundant in all filters. Clade B comammox comprise the majority (∼75%) of comammox abundance in all filters. Nitrosomonadaceae were present in all filters, although at low abundance (mean = 1.8%). Ordination suggests that temperature impacts the structure of nitrifying communities, and in particular that increasing temperature favours Nitrospira. The nitrogen content of the filter material, sulfate concentration and surface ammonium loading rates shape the structure of the comammox guild in the filters. This work provides an assay for simultaneous detection and diversity assessment of clades A and B comammox Nitrospira, expands our current knowledge of comammox Nitros...Continue Reading

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Citations

Aug 24, 2018·FEMS Microbiology Ecology·Albin AlfreiderMonika Summerer
Oct 14, 2018·Applied and Environmental Microbiology·Fei XiaZhe-Xue Quan
Jan 29, 2020·Journal of Applied Microbiology·Y XuJ Zhao
Nov 12, 2018·Applied Microbiology and Biotechnology·Hanna KochSebastian Lücker
Feb 16, 2019·Frontiers in Microbiology·Natalie K Beach, Daniel R Noguera
Aug 28, 2020·Frontiers in Microbiology·Yu TakahashiSatoshi Tsuneda
Oct 16, 2020·The Science of the Total Environment·Yuxiang ZhaoBaolan Hu
Jan 16, 2021·Journal of Basic Microbiology·Ardhra VijayanBright Singh Isaac Sarojini
Feb 6, 2021·Current Microbiology·Julia HeiseRainer U Meckenstock
Apr 6, 2021·Water Research·Yulin WangTong Zhang
Apr 28, 2021·Environmental Science & Technology·Guoqiang LiuLi Zhuang
May 30, 2021·Water Research·Shufeng LiuJinren Ni
Jul 29, 2021·The ISME Journal·Man-Young JungMichael Wagner
Aug 15, 2021·The ISME Journal·Xiangxin SunZhongjun Jia
Aug 21, 2021·Marine Biotechnology·Surachet Burut-ArchanaiSanit Piyapattanakorn

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