Combination antibiotic supplementation of corneal storage medium

American Journal of Ophthalmology
D G HwangT M Smith

Abstract

Gram-positive cocci frequently contaminate donor corneal tissue and represent the most common cause of postkeratoplasty endophthalmitis. Although gentamicin is currently added to corneal storage medium in an effort to decrease bacterial contamination of donor tissue, it has poor or variable in vitro activity against many strains of streptococci and staphylococci. To investigate whether the antibiotic supplementation of corneal storage media could be improved, we surveyed 11 antibiotics for antimicrobial efficacy under simulated storage conditions against gentamicin-resistant strains of Staphylococcus aureus, S. epidermidis, Streptococcus pneumoniae, and St. viridans. All antibiotics showed markedly reduced activity at 4 C as compared to their predicted activity at 37 C. Bactericidal activity of streptomycin and tobramycin was enhanced by preceding 4 C storage with a three-hour period at room temperature (23 C). Under these conditions, streptomycin showed the best antimicrobial activity of the 11 antibiotics tested. Addition of gentamicin to streptomycin resulted in further improvement of activity against S. aureus and S. epidermidis, whereas the addition of penicillin G to streptomycin enhanced the activity against St. viridans...Continue Reading

References

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Citations

Jul 1, 1997·American Journal of Ophthalmology·Y C Hou, F R Hu
Nov 11, 2009·Current Eye Research·Davi Chen WuRichard Yudi Hida
Dec 29, 1998·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·E RaposioP Santi
Aug 11, 2006·Current Opinion in Ophthalmology·Bennie H Jeng
Aug 22, 2008·Ocular Immunology and Inflammation·Kuan-Jen ChenYen-Po Chen
Nov 4, 2010·Cornea·Thomas D LindquistMonty Montoya
Dec 20, 2018·The British Journal of Ophthalmology·Nicola LauStephen J Tuft

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