Combination effects of epidermal growth factor and glial cell line-derived neurotrophic factor on the in vitro developmental potential of porcine oocytes

Zygote : the Biology of Gametes and Early Embryos
Mehdi Vafaye VallehPoul Hyttel

Abstract

The developmental potential of in vitro matured porcine oocytes is still lower than that of oocytes matured and fertilized in vivo. Major problems that account for the lower efficiency of in vitro production include the improper nuclear and cytoplasmic maturation of oocytes. With the aim of improving this issue, the single and combined effects of epidermal growth factor (EGF) and glial cell line-derived neurotrophic factor (GDNF) on oocyte developmental competence were investigated. Porcine cumulus-oocyte cell complexes (COCs) were matured in serum-free medium supplemented with EGF (0, 10 or 50 ng/ml) and/or GDNF (0, 10 or 50 ng/ml) for 44 h, and subsequently subjected to fertilization and cultured for 7 days in vitro. The in vitro-formed blastocysts derived from selected growth factor groups (i.e. EGF = 50 ng/ml; GDNF = 50 ng/ml; EGF = 50 ng/ml + GDNF = 50 ng/ml) were also used for mRNA expression analysis, or were subjected to Hoechst staining. The results showed that the addition of EGF and/or GDNF during oocyte maturation dose dependently enhanced oocyte developmental competence. Compared with the embryos obtained from control or single growth factor-treated oocytes, treatment with the combination of EGF and GDNF was shown ...Continue Reading

References

May 1, 1991·The Journal of Cell Biology·C L RawsonD W Barnes
Sep 1, 1994·Molecular Reproduction and Development·J Ding, G R Foxcroft
Mar 1, 1996·The Journal of Biological Chemistry·J BaulidaG Carpenter
Jan 1, 1996·Reproduction, Fertility, and Development·J J Eppig
May 14, 1998·Chemistry & Biology·N A Thornberry
Aug 28, 1998·Science·N A Thornberry, Y Lazebnik
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·J WangN G Larsson
Jul 27, 2001·Theriogenology·R MarchalP Mermillod
Feb 9, 2002·The Journal of Endocrinology·K Hardy, S Spanos
Sep 5, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Henry J Leese
Apr 18, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Sep 23, 2003·Molecular Reproduction and Development·Xiang-Shun Cui, Nam-Hyung Kim
Feb 18, 2004·The Journal of Reproduction and Development·Qing-Yuan Sun, Takashi Nagai
Mar 16, 2004·Human Molecular Genetics·Mats I EkstrandNils-Göran Larsson
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sonja SchaetzleinK Lenhard Rudolph
Aug 3, 2004·Science·Douglas R Green, Guido Kroemer
Mar 1, 2005·Molecular Reproduction and Development·Gab Sang LeeWoo Suk Hwang
May 10, 2005·Molecular Human Reproduction·G CauffmanA Van Steirteghem
Jun 30, 2005·Zygote : the Biology of Gametes and Early Embryos·Yu-Jeong JeongNam-Hyung Kim
Jul 30, 2005·Molecular Endocrinology·Marco ContiYou-Qiang Su
Dec 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Lisa M Mehlmann
Dec 22, 2007·Nature Reviews. Molecular Cell Biology·Richard J Youle, Andreas Strasser
Jan 18, 2008·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Feb 16, 2008·The Journal of Reproduction and Development·Frank Dean JousanPeter James Hansen
May 8, 2008·Current Opinion in Obstetrics & Gynecology·Kevin S Richter
Jan 21, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Rhiannon E LloydPilar Coy
Mar 3, 2009·Advanced Drug Delivery Reviews·Bernadett Kalmar, Linda Greensmith
Mar 19, 2009·The Journal of Reproduction and Development·Ken-Ichi YamanakaEimei Sato

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