Abstract
Recent efforts to treat malignancy using gene transfer have met with varying degrees of success. In this paper, we report the results of studies using two recombinant adenoviral vectors to examine the efficacy of combination gene transfer to cause tumor regression in vivo. One of these vectors encodes the murine MHC class I gene, H-2Kb (ADV-Kb), which induces an immune response that stimulates tumor regression. The second vector encodes the human p21 cyclin dependent kinase inhibitor (ADV-p21). This gene product arrests cell cycle progression and prevents proliferation of tumor cells. Both vectors were tested in a murine model in vivo for antitumor effect. As previously shown, a significant reduction of tumor size was observed with each vector. Combination treatment, in which both vectors were administered, resulted in a trend toward a reduced tumor growth greater than with either vector alone. In order to characterize the mechanism of tumor regression, cytolytic T lymphocyte (CTL) assays against the allogeneic molecule, H-2Kb, were performed. Mice treated with ADV-Kb showed specific CTL activity against the H-2Kb molecule, demonstrating that the immune response against the H-2Kb gene product involved in tumor regression was po...Continue Reading
Citations
Sep 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G S Crary, J H Albrecht
Jun 15, 2011·Journal of Biomolecular NMR·Mallika SastryPeter D Kwong
Jan 14, 1998·Nature Medicine·L XuG J Nabel
Apr 29, 1998·Human Gene Therapy·B M PützerF L Graham
Dec 5, 1998·Journal of the National Cancer Institute·A N RakkarP Seth
May 7, 2002·Journal of Virology·Bimal K ChakrabartiGary J Nabel
Apr 20, 1999·Oncogene·M SchreiberF L Graham
Sep 7, 1999·Gene Therapy·M C KeoghN R Lemoine
Apr 21, 2001·Oncogene·W YangG E Sonenshein
Apr 21, 2001·Oncogene·R SugibayashiK Takeda
Mar 19, 2019·Circulation Research·Ye FanQingbo Xu
Dec 26, 2001·Oncogene·G D BrenC V Paya
May 18, 2019·Circulation Research·Kyuho JeongSsang-Taek Steve Lim
Sep 14, 2004·Oncogene·Jinghai Wu, Jerry B Lingrel
Oct 10, 1998·Journal of Virology·X DanthinneE G Nabel