Combination of adrenomedullin with its binding protein accelerates cutaneous wound healing

PloS One
Juan-Pablo IdrovoPing Wang

Abstract

Cutaneous wound continues to cause significant morbidity and mortality in the setting of diseases such as diabetes and cardiovascular diseases. Despite advances in wound care management, there is still an unmet medical need exists for efficient therapy for cutaneous wound. Combined treatment of adrenomedullin (AM) and its binding protein-1 (AMBP-1) is protective in various disease conditions. To examine the effect of the combination treatment of AM and AMBP-1 on cutaneous wound healing, full-thickness 2.0-cm diameter circular excision wounds were surgically created on the dorsum of rats, saline (vehicle) or AM/AMBP-1 (96/320 μg kg BW) was topically applied to the wound daily and wound size measured. At days 3, 7, and 14, skin samples were collected from the wound sites. AM/AMBP-1 treated group had significantly smaller wound surface area than the vehicle group over the 14-day time course. At day 3, AM/AMBP-1 promoted neutrophil infiltration (MPO), increased cytokine levels (IL-6 and TNF-α), angiogenesis (CD31, VEGF and TGFβ-1) and cell proliferation (Ki67). By day 7 and 14, AM/AMBP-1 treatment decreased MPO, followed by a rapid resolution of inflammation characterized by a decrease in cytokines. At the matured stage, AM/AMBP-1 ...Continue Reading

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Citations

Nov 26, 2015·Molecular Immunology·Robert B SimUday Kishore
Feb 6, 2017·Journal of Peptide Science : an Official Publication of the European Peptide Society·Ria SchönauerAnnette G Beck-Sickinger
Jan 9, 2016·International Journal of Molecular Medicine·Juan Pablo IdrovoPing Wang
Jan 10, 2016·Biological Research for Nursing·Eva L ArantesEliana P Araújo
Apr 14, 2018·Tissue Engineering. Part a·Byeong-Wook SongHoon-Bum Lee
Jan 24, 2020·Scientific Reports·Ayşe KılıçScott T Weiss
Dec 4, 2019·Autonomic Neuroscience : Basic & Clinical·Georgios Theocharidis, Aristidis Veves

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Methods Mentioned

BETA
PCR
light microscopy

Software Mentioned

NIH ImageJ
NIH Image J

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