PMID: 8955511Nov 1, 1996Paper

Combination of antiviral immunotoxin and ganciclovir or cidofovir for the treatment of murine cytomegalovirus infections

Antiviral Research
D F SmeeB B Barnett

Abstract

The effects of two anti-murine cytomegalovirus (MCMV) immunotoxins used in combination with ganciclovir (GCV) or cidofovir (HPMPC) against MCMV were determined in vitro and in mice. The inhibitors were added to cell cultures 24 or 48 h after MCMV adsorption so as to not affect the initial infection rate. The immunotoxins (0.63, 1.25 and 2.5 micrograms/ml) combined with GCV (1.25, 2.5 and 5 microM) or HPMPC (0.03, 0.06 and 0.12 microM) caused synergistic inhibition of virus yield in C127I cells at most of the combinations tested. No toxic effect on cell growth in culture was observed at these immunotoxin/drug combinations. The effects of immunotoxin and GCV treatment were studied further in MCMV-infected severe combined immunodeficient (SCID) mice. Immunotoxin (1 mg/kg per day) given by intraperitoneal (i.p.) injection on days 1, 4 and 7 of the infection did not extend the mean day to death compared with the placebo group. Once daily i.p. treatment with GCV (50 mg/kg per day) for days starting at 24 h after virus inoculation extended survival time almost 11 days. The combination of immunotoxin plus GCV was better than GCV alone, extending the mean day to death an additional 2 to 3 days, which is suggestive of a synergistic effect.

References

Oct 1, 1990·Antiviral Research·M N Prichard, C Shipman
Sep 1, 1990·Reviews of Infectious Diseases·R H Rubin
Sep 1, 1990·Reviews of Infectious Diseases·R T Schooley
Feb 1, 1989·The Journal of Infectious Diseases·M D Yow
Mar 1, 1988·The Journal of Infectious Diseases·S L WalmsleyM M Fanning

❮ Previous
Next ❯

Citations

Jan 6, 2016·Journal of Leukocyte Biology·Katja SpiessMette M Rosenkilde
Jan 28, 2021·Toxins·Lucía CitoresJosé M Ferreras

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.