Abstract
Three in vitro studies were designed to develop an assay for anticoccidial efficacy by use of laboratory (Houghton) and field (T-376) Eimeria tenella strains. In study (1), minimum inhibitory concentrations (MICs) of monensin (Mon), maduramicin (Mad), salinomycin (Sal), and lasalocid (Las) were determined that are able to inhibit more than 50% of sporozoites in host cell (Madin-Darby bovine kidney (MDBK)) penetration and more than 95% of Houghton sporozoites development to mature merozoites (treatment time 24 h) using quantitative real-time PCR (qPCR). MICs were 0.5, 2.5, 1, and 0.5 μg/ml for Mon, Mad, Sal, and Las, respectively. Applying the previous MIC on T-376 strain revealed a different sensitivity profile. Mad reduced T-376 gene copies by only 89.3% after 96 h of infection. In study (2), Houghton strain sporozoites were incubated with MIC of the different tested ionophores for 2 and 4 h, respectively; afterwards, their ability to invade MDBK cells was determined using phase-contrast microscopy and qPCR. Treatment of sporozoites with ionophores for 4 h resulted in significant inhibition of invasion compared with non-treated parasites as assessed both by microscopy as well as qPCR. Inhibition rates for Mon, Mad, Sal, and La...Continue Reading
References
Dec 1, 1976·Experimental Parasitology·L R McDougald, R B Galloway
Feb 1, 1986·The Journal of protozoology·D M SchmatzP K Murray
Aug 1, 1970·Experimental Parasitology·J Johnson, W M Reid
Jun 1, 1980·Parasitology·M A Peirce
Feb 1, 1984·The Journal of protozoology·D M SchmatzP K Murray
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jan 1, 1983·Zeitschrift Für Parasitenkunde·H MehlhornW Raether
Apr 1, 1997·Veterinary Parasitology·B StephenA Haberkorn
Sep 2, 1998·International Journal for Parasitology·H D Chapman
Jan 10, 2002·Clinical Microbiology Reviews·P C Allen, R H Fetterer
Jul 31, 2002·Applied and Environmental Microbiology·Paul A RochelleRicardo De Leon
Apr 10, 2003·Parasitology Research·J Tierney, G Mulcahy
Dec 13, 2006·The Journal of Parasitology·Zhuo WangJianzhong Shen
Jun 26, 2007·Avian Pathology : Journal of the W.V.P.A·H W Peek, W J M Landman
Jan 27, 2009·Avian Diseases·Fumiya KawaharaTetsuo Nunoya
Oct 15, 2014·Veterinary Parasitology·M C JenkinsR Fetterer
Citations
Aug 13, 2015·Parasitology Research·Alaa Aldin AlnassanBerit Bangoura
Jul 26, 2012·PloS One·Lianlian JiangBing Huang
Aug 8, 2018·Parasites & Vectors·Runhui ZhangBerit Bangoura
Jan 17, 2020·Parasitology Research·Zaida Rentería-SolísArwid Daugschies
Feb 13, 2017·Parasitology Research·Ane OddenSnorre Stuen
Aug 28, 2020·Frontiers in Veterinary Science·Erasmia SidiropoulouAthina Tzora
Oct 9, 2020·Parasitology Research·Runhui ZhangBerit Bangoura
Nov 7, 2020·Frontiers in Cellular and Infection Microbiology·Virginia Marugan-HernandezFiona Tomley
Jun 20, 2021·Parasitology Research·Shahinaz TahaZaida Rentería-Solís
Nov 11, 2021·Parasitology Research·Ahmed ThabetBerit Bangoura