Combination of curcumin and piperine prevents formation of gallstones in C57BL6 mice fed on lithogenic diet: whether NPC1L1/SREBP2 participates in this process?

Lipids in Health and Disease
Yongnan LiYu Tian

Abstract

A disruption of cholesterol homeostasis characterized by the physical-chemical imbalance of cholesterol solubility in bile often results in formation of cholesterol gallstones. Our earlier studies revealed that curcumin (1000 mg/kg) could prevent formation of gallstones. It has been proved that curcumin is poorly absorbed while piperine is a bioavailability-enhancer. Nevertheless, whether curcumin combined with piperine could enhance the effect of curcumin in preventing gallstones is still awaited. C57BL6 mice were fed on a lithogenic diet concomitant with curcumin at 500 or 1000 mg/kg and/or piperine at 20 mg/kg for 4 weeks. The ratio of gallbladder stone formation was recorded and samples of blood, bile, gallbladder, liver and small intestine were also collected. The volume of gallbladder and weight of liver were calculated, and blood and bile samples were analyzed through biochemical methods. Intestinal NPC1L1 and SREBP2 mRNA and protein expression were detected by real-time PCR and Western blot. Combining with piperine can significantly enhance the effect of curcumin, thus preventing the development of gallbladder stones, lowering the saturation of blood lipids and cholesterol in bile, as well as decreasing the expression o...Continue Reading

References

Jan 13, 1995·The Journal of Biological Chemistry·T W Loo, D M Clarke
Jun 13, 2003·Atherosclerosis·Peggy RobinetJean Louis Paul
Sep 28, 2006·Annual Review of Physiology·David Q-H Wang
Sep 30, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Waddah A AlrefaiRavinder K Gill
Nov 30, 2006·Journal of Lipid Research·Dawn E TelfordMurray W Huff
Jan 16, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Harry R DavisScott W Altmann
Apr 4, 2007·Annals of the New York Academy of Sciences·Viktor JefremovEllo Karelson
Jun 16, 2007·Advances in Experimental Medicine and Biology·Ricky A SharmaAndreas J Gescher
Mar 8, 2008·Cellular and Molecular Life Sciences : CMLS·H HatcherS V Torti
Mar 13, 2008·The Journal of Antimicrobial Chemotherapy·Ashwani KumarGhulam Nabi Qazi
Apr 30, 2008·Gastroenterology·Helen H WangDavid Q-H Wang
Jan 15, 2009·Journal of Lipid Research·Ryan E TemelLiqing Yu
Apr 30, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Pradeep KumarWaddah A Alrefai
Jun 28, 2012·Journal of Natural Medicines·Acharaporn DuangjaiNanteetip Limpeanchob
Jun 20, 2014·Experimental and Therapeutic Medicine·Yaosheng TuYuxing Chen
Nov 30, 2014·BMC Complementary and Alternative Medicine·Mohamed Mohamed SolimanTamer Ahmed Ismail

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Citations

Jan 12, 2019·Critical Reviews in Food Science and Nutrition·Sita Sharan PatelPriyal Jain
Nov 25, 2020·Thyroid : Official Journal of the American Thyroid Association·Irina KubeDenise Zwanziger
Nov 17, 2020·Frontiers in Veterinary Science·Ye SunYang Bai
Sep 21, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wang LiaoYan Ding
Jan 25, 2021·Liver International : Official Journal of the International Association for the Study of the Liver·Eva GijbelsLindsey Devisscher

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Methods Mentioned

BETA
PCR
protein assay

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