PMID: 25744845Mar 7, 2015Paper

Combination of cytosine arabinoside and cisplatin enhances inhibition of cell proliferation and promotes apoptosis of resistant nasopharyngeal carcinoma cells

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Jianjun ChenJie Peng

Abstract

To investigate the synergistic cytotoxicity of cisplatin and cytosine arabinoside (Ara-C) on nasopharyngeal carcinoma (NPC) cell lines TW03 and cisplatin-resistant TW03/DDP which is cisplatin-induced drug-resistant cell line. The TW03/DDP cells was established by gradually increasing the dose of cisplatin, and then examined by real-time quantitative PCR (qRT-PCR) and semi-quantitative Western blotting for the expression level of lung resistance-related protein (LRP). Effects of DDP and Ara-C combined treatment or alone on proliferation and apoptosis of TW03/DDP cells and TW03 cells were detected by CCK-8 assay and flow cytometry, respectively. Compared with TW03 cells, TW03/DDP cells expressed higher level of LRP. After treatments for 24 hours, both cisplatin and Ara-C inhibited proliferation and caused apoptosis of TW03/DDP and TW03 cells, and DDP showed minor effect on the proliferation of TW03/DDP cells. Compared with Ara-C or DDP treatment alone, combination of Ara-C and DDP showed a more remarkable inhibition of proliferation and promotion of apoptosis in both TW03 and TW03/DDP cells. Ara-C and DDP have a synergistic killing effect on NPC cell lines with and without DDP-caused drug resistance, and show better effects on pr...Continue Reading

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