Combination of electroacupuncture and grafted mesenchymal stem cells overexpressing TrkC improves remyelination and function in demyelinated spinal cord of rats

Scientific Reports
Ying DingYuan-Shan Zeng

Abstract

This study attempted to graft neurotrophin-3 (NT-3) receptor (TrkC) gene modified mesenchymal stem cells (TrkC-MSCs) into the demyelinated spinal cord and to investigate whether electroacupuncture (EA) treatment could promote NT-3 secretion in the demyelinated spinal cord as well as further enhance grafted TrkC-MSCs to differentiate into oligodendrocytes, remyelination and functional recovery. Ethidium bromide (EB) was microinjected into the spinal cord of rats at T10 to establish a demyelinated model. Six groups of animals were prepared for the experiment: the sham, PBS, MSCs, MSCs+EA, TrkC-MSCs and TrkC-MSCs+EA groups. The results showed that TrkC-MSCs graft combined with EA treatment (TrkC-MSCs+EA group) significantly increased the number of OPCs and oligodendrocyte-like cells differentiated from MSCs. Immunoelectron microscopy showed that the oligodendrocyte-like cells differentiated from TrkC-MSCs formed myelin sheaths. Immunofluorescence histochemistry and Western blot analysis indicated that TrkC-MSCs+EA treatment could promote the myelin basic protein (MBP) expression and Kv1.2 arrangement trending towards the normal level. Furthermore, behavioural test and cortical motor evoked potentials detection demonstrated a signi...Continue Reading

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Citations

Sep 26, 2020·Frontiers in Cell and Developmental Biology·Patricia Kauanna Fonseca DamascenoMilena Botelho Pereira Soares
Oct 7, 2015·Stem Cell Research & Therapy·Bharath ChelluboinaKrishna Kumar Veeravalli
Nov 8, 2017·Neuromodulation : Journal of the International Neuromodulation Society·Ling-Yong XiaoCun-Zhi Liu
Sep 27, 2018·Gene Therapy·Wei WeiWei Wang

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Methods Mentioned

BETA
ELISA
fluorescence microscopy
transgenic
enzyme-linked immunosorbent assay
electron microscopy
transmission electron microscopy
electrophoresis

Software Mentioned

ZEN
ImageJ
NIH
SPSS17

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