Combination of IFITM1 knockdown and radiotherapy inhibits the growth of oral cancer

Cancer Science
Jie YangXiaojiang Li

Abstract

This research aimed to analyze the effect of IFITM1 on the radioresistance of oral neoplasm. Using a multi-group heat map from GSE9716 analysis of the GEO database, IFITM1 was determined to be a relevant radioresistance gene. The TCGA database was analyzed before the expression of IFITM1 was analyzed. IFITM1 expression was quantified by quantitative RT-PCR and immunohistochemistry in 19 paired oral neoplasm cases. The effects of time and dose of radiation on IFITM1 expression level in CAL27 and TSCC1 cell lines were tested by quantitative RT-PCR. Oral neoplasm cells were transfected with siRNA after radiotherapy to disturb IFITM1 expression. After this, the survival rates, cell apoptosis, caspase-3 viability, expression and γ-H2AX were detected using colony formation, flow cytometry, western blot and immunofluorescence, respectively. Western blot was used for STAT1/2/3/p21-related protein and phosphorylation changes. Finally, an in vivo nude mice tumor model was established to verify the effect of IFITM1 on oral neoplasm cells radioresistance. Through microarray analysis, the head and neck neoplasm radioresistance-related gene IFITM1 was found to be overexpressed. IFITM1 overexpression was verified not only using the TCGA datab...Continue Reading

References

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Citations

May 13, 2020·Molecular Diagnosis & Therapy·Renba LiangXiaodong Zhu
Feb 7, 2021·Journal of Cancer Research and Clinical Oncology·Yanbei KuangQiang Li
Apr 2, 2021·Omics : a Journal of Integrative Biology·Firdous Ahmad BhatAditi Chatterjee

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Datasets Mentioned

BETA
GSE9716

Methods Mentioned

BETA
surgical resection
transfection
FCS
Flow cytometry
xenograft

Software Mentioned

GraphPad
R
Step One
GraphPad Prism
Image J

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