Combination of in vivo angiopoietin-1 gene transfer and autologous bone marrow cell implantation for functional therapeutic angiogenesis

Arteriosclerosis, Thrombosis, and Vascular Biology
Koichi KobayashiToyoaki Murohara

Abstract

Autologous bone marrow mononuclear cell (BM-MNC) implantation into ischemic tissues promotes angiogenesis, but a large amount of marrow aspiration is required, which is a major clinical limitation. Angiopoietin-1 (Ang-1) is requisite for vascular maturation during angiogenesis. We examined the impacts of combinatorial Ang-1 gene transfer and low-dose autologous BM-MNC implantation on therapeutic angiogenesis in a rabbit model of hind limb ischemia. Rabbits were divided into 4 groups: phosphate-buffered saline (control), 500 microg Ang-1 plasmid (Ang-1), 1 x 10(6) autologous BM-MNCs (BMC), and Ang-1 plasmid plus BM-MNCs (combination). The Ang-1 group had a greater angiographic score and capillary density compared with the control (P<0.05), but the Ang-1 gene therapy alone did not improve transcutaneous oxygen pressure (TcO2) and skin ulcer score. However, the combination group showed a significant improvement in not only angiographic score and capillary density (P<0.05) but also TcO2 (P<0.05) and skin ulcer score. These efficacies were greater in the combination group compared with the BMC group. This Ang-1 gene and BM-MNC combination therapy enhances not only quantitative but also qualitative angiogenesis in ischemic tissues. M...Continue Reading

References

May 20, 1998·Current Biology : CB·T I KoblizekW Risau
Jun 17, 1998·The Journal of Clinical Investigation·T MuroharaJ M Isner
Oct 17, 1998·Science·C SuriG D Yancopoulos
Dec 9, 1998·Molecular Cell·M L SpringerH M Blau
Mar 29, 2000·The Journal of Biological Chemistry·A PapapetropoulosW C Sessa
Mar 31, 2000·Nature Medicine·P Carmeliet
Mar 31, 2000·Nature Medicine·G ThurstonG D Yancopoulos
Jun 7, 2000·The Journal of Clinical Investigation·T MuroharaT Imaizumi
May 1, 2001·Nature Medicine·H M Blau, A Banfi
Sep 21, 2002·Lancet·Eriko Tateishi-YuyamaUNKNOWN Therapeutic Angiogenesis using Cell Transplantation (TACT) Study Investigators
Jan 21, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Takako AkitaTsutomu Imaizumi
Feb 14, 2003·The New England Journal of Medicine·Jonathan M HillToren Finkel
Mar 19, 2003·Circulation·Timothy D HenryUNKNOWN VIVA Investigators
Jun 11, 2003·American Heart Journal·Sanjay RajagopalanUNKNOWN Regional Angiogenesis With Vascular Endothelial Growth Factor trial
Feb 7, 2004·Blood·Yuichi OikeToshio Suda
Apr 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chung-Hyun ChoGou Young Koh

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Citations

Aug 21, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Xabier L ArangurenAernout Luttun
Oct 13, 2007·Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·Toyoaki Murohara
Mar 6, 2007·Nihon Ronen Igakkai zasshi. Japanese journal of geriatrics·Takahisa KondoYasushi Numaguchi
Aug 21, 2007·Expert Opinion on Biological Therapy·Gabriel ZarbivMoshe Y Flugelman
Apr 29, 2014·Expert Opinion on Biological Therapy·Munehisa ShimamuraRyuichi Morishita
Feb 27, 2017·Journal of Translational Medicine·Makoto SamuraKimikazu Hamano
Apr 7, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Sahoko IchiharaToyoaki Murohara
Aug 28, 2009·Journal of Cellular Biochemistry·Erica B SneiderLouis M Messina
Nov 12, 2009·Cardiovascular Therapeutics·Antonia GermaniMaurizio C Capogrossi
Dec 20, 2015·American Journal of Physiology. Heart and Circulatory Physiology·Luqia HouNgan F Huang
Jul 24, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Cheng-Huang SuHung-I Yeh
Jun 22, 2019·Expert Review of Cardiovascular Therapy·Marco MiceliClaudio Napoli
Aug 12, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Yasutaka YamamotoIchiro Hisatome

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