Combination of insulin-like growth factor (IGF)-I and IGF-binding protein-1 promotes fibroblast-embedded collagen gel contraction

Endocrinology
Y R LeeH Ogawa

Abstract

Wound contraction is an important event that minimizes the wound defect during the healing process. Involvement of insulin-like growth factor (IGF)-I and IGF-binding protein (IGFBP)-1 in wound contraction was studied using an in vitro model. Human dermal fibroblasts (1 x 10(5) cells/ml) were incorporated into a porcine type I collagen (0.21% final) in serum-free medium. The fibroblast-embedded collagen gels in a 12-well plate were floated from the well, and various reagents were then added to the assay medium. The surface area of the gel was calculated by measuring the diameters of the collagen gel. IGF-I at high doses (30 and 100 ng/ml) revealed 6.8% (P < 0.01) and 7.7% (P < 0.001) gel contraction, respectively, and des (1-3) IGF-I at 10 ng/ml produced a 4.5% gel contraction (P < 0.01). Meanwhile, IGFBP-I did not induce any significant contraction throughout the tested concentrations (0.1-100 ng/ml). A combination of IGF-I and IGFBP-1 at 1 ng/ml of each reagent, a concentration at which gel contraction was not observed when each of the reagents was tested individually, produced a 14% gel contraction (P < 0.001), whereas combinations of des (1-3) IGF-I with IGFBP-1 at the same concentrations did not promote gel contraction. The...Continue Reading

Citations

Jun 17, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Oyvind Sverre SvendsenRolf K Reed
Jul 25, 2006·Archives of Dermatological Research·Hiroshi NakaoHideoki Ogawa
Oct 29, 2011·Archives of Dermatological Research·Masuyoshi SaitoRyoji Tsuboi
May 31, 2008·International Urogynecology Journal and Pelvic Floor Dysfunction·S MeyerL Juillerat-Jeanneret
Jan 9, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Natalie M MorelliniMark W Fear
Jan 8, 2009·International Journal of Dermatology·Yuki MorokumaRyoji Tsuboi
Feb 6, 2002·Bioscience, Biotechnology, and Biochemistry·Y TakayamaK Mizumachi
Jun 28, 2005·Journal of Dermatological Science·Toshio HasegawaHideoki Ogawa
Jul 25, 2000·The British Journal of Dermatology·K B SuhrH Ogawa
Dec 6, 2002·Endocrine Reviews·Sue M Firth, Robert C Baxter
Sep 11, 2003·Journal of Wound Care·R Tejero-Trujeque
Dec 13, 2003·Endocrine Reviews·Stephanie R EdmondsonChristopher J Wraight
Oct 29, 2000·Cell Adhesion and Communication·S KanekarW Carver
May 21, 1999·The Journal of Surgical Research·K M BullardM J Banda
Jun 12, 2004·The Journal of Investigative Dermatology·Koji SumiyoshiHideoki Ogawa
Feb 8, 2005·American Journal of Obstetrics and Gynecology·Sébastien PoncetLucienne Juillerat-Jeanneret
Nov 28, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Jacob C ZbindenJenny C Barker

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