Combination of magnesium ions and vitamin C alleviates synovitis and osteophyte formation in osteoarthritis of mice

Bioactive Materials
Hao YaoLing Qin

Abstract

We previously demonstrated that magnesium ions (Mg2+) was a novel therapeutic alternative for osteoarthritis (OA) through promoting the hypoxia inducible factor-1α (HIF-1α)-mediated cartilage matrix synthesis. However, oxidative stress can inhibit the expression of HIF-1α, amplify the inflammation that potentially impairs the therapeutic efficacy of Mg2+ in OA. Vitamin (VC), a potent antioxidant, may enhance the efficacy of Mg2+ in OA treatment. This study aims to investigate the efficacy of combination of Mg2+ and VC on alleviating joint destruction and pain in OA. Anterior cruciate ligament transection with partial medial meniscectomy induced mice OA model were randomly received intra-articular injection of either saline, MgCl2 (0.5 mol/L), VC (3 mg/ml) or MgCl2 (0.5 mol/L) plus VC (3 mg/ml) at week 2 post-operation, twice weekly, for 2 weeks. Joint pain and pathological changes were assessed by gait analysis, histology, western blotting and micro-CT. Mg2+ and VC showed additive effects to significantly alleviate the joint destruction and pain. The efficacy of this combined therapy could sustain for 3 months after the last injection. We demonstrated that VC enhanced the promotive effect of Mg2+ on HIF-1α expression in cartila...Continue Reading

References

May 29, 1998·Journal of Molecular and Cellular Cardiology·B K Slinker
Mar 17, 2004·Osteoarthritis and Cartilage·Ibsen B CoimbraDavid G Stokes
Jun 7, 2005·Osteoarthritis and Cartilage·Y HenrotinT Aigner
Dec 14, 2007·The Journal of Biological Chemistry·Jérôme E LafontChristopher L Murphy
Mar 3, 2010·Arthritis Research & Therapy·Simon StröbelIvan Martin
Jun 9, 2010·Nature Medicine·Matthew HusaRobert Terkeltaub
Oct 7, 2010·Nature Reviews. Rheumatology·Jérémie Sellam, Francis Berenbaum
Dec 2, 2010·Nature Reviews. Rheumatology·Mohit KapoorHassan Fahmi
Jan 27, 2011·The Journal of Bone and Joint Surgery. American Volume·Maryfran SowersMatheos Yosef
Nov 2, 2011·Arthritis and Rheumatism·Alfred OpolkaSusanne Grässel
Aug 15, 2012·Osteoarthritis and Cartilage·S C FuK M Chan
Jan 22, 2013·Arthritis and Rheumatism·Brendan L ThomsChristopher L Murphy
Mar 26, 2014·Nature Reviews. Rheumatology·Hang Fang, Frank Beier
Jul 27, 2014·Matrix Biology : Journal of the International Society for Matrix Biology·Tanja NiedermairSusanne Grässel
Jan 3, 2015·Joint, Bone, Spine : Revue Du Rhumatisme·Fang-Jie ZhangGuang-Hua Lei
Jan 22, 2015·Arthritis Research & Therapy·Hans-Georg Schaible
Jun 3, 2015·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Kirk A CampbellBrian J Cole
Sep 12, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Patrick AghajanianSubburaman Mohan
Oct 21, 2016·Osteoarthritis and Cartilage·Emily H Lakes, Kyle D Allen
Apr 29, 2016·Proceedings of the National Academy of Sciences of the United States of America·Wafa BouazizEric Haÿ
Nov 5, 2016·Science·Ru-Rong JiYu-Qiu Zhang
Dec 31, 2016·International Journal of Molecular Sciences·Pu-Rong ChiuKee-Lung Chang
Apr 7, 2017·Nature Reviews. Rheumatology·Ali MobasheriUrsula Fearon
Jan 18, 2018·Trends in Immunology·Andrew D CookJohn A Hamilton
Apr 18, 2018·Journal of Orthopaedic Translation·Ling KongKevin K W Ho
May 2, 2018·Osteoarthritis and Cartilage·F P B KroonM Kloppenburg
May 2, 2018·The Journal of Pain : Official Journal of the American Pain Society·Tian-Zhi GuoJohn David Clark
Dec 12, 2018·The Journal of Clinical Investigation·Shouan ZhuXu Cao
Jan 30, 2019·International Journal of Molecular Sciences·Paola CastrogiovanniGiuseppe Musumeci
Feb 13, 2019·Annals of the Rheumatic Diseases·Wenxue TongKingston King-Lun Mak

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

GraphPad Prism
ARRIVE
Catwalk XT
Catwalk
OARSI

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Loïc LouvetZiad Massy
Biochemical and Biophysical Research Communications
Akinori KashioTatsuya Yamasoba
Asian-Australasian Journal of Animal Sciences
T Matsui
Kokyu to junkan. Respiration & circulation
S Suetsugu
© 2021 Meta ULC. All rights reserved