Combination of PCR targeting the VD2 of omp1 and reverse line blot analysis for typing of urogenital Chlamydia trachomatis serovars in cervical scrape specimens

Journal of Clinical Microbiology
Monica MolanoAdriaan J C van den Brule

Abstract

In this study we developed and evaluated a new PCR-based typing assay, directed to the VD2 region of the omp1 gene, for the detection and typing of urogenital Chlamydia trachomatis infections. A nested VD2 PCR-reverse line blot (RLB) assay was developed for the typing of nine different urogenital serovars of C. trachomatis. The assay developed was tested with reference strains of C. trachomatis serovars and cervical scrapes of 86 Colombian women previously found to be positive for C. trachomatis by using plasmid PCR. Two sets of primers directed to the VD2 region of the omp1 gene of C. trachomatis were designed, and fragments of 220 and 166 bp were generated in the primary and nested PCRs, respectively. In addition, an RLB assay was developed to identify nine different urogenital serovars of C. trachomatis (Ba, D, E, F, G, H, I, J, and K) and group controls, including group B (Ba, D, and E), group C (I, J, K, and H), and an intermediate group (F and G). Using this assay, we were able to type 81 of the 86 samples (94.2%). Of these samples, 91.3% were single C. trachomatis infections, and 8.7% were multiple infections. The most common serovars identified were serovars D (22.2%), F (18.5%), G (13.6%), and E (12.3%). Of the women w...Continue Reading

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Citations

Jan 6, 2009·Current Microbiology·Jialin YuLinyan Hu
Apr 7, 2007·Sexually Transmitted Diseases·Haleta E LimaFátima Soares Motta Noronha
Jul 19, 2006·Clinical Microbiology Reviews·Aparajita SinghMarcus J Zervos
Jun 15, 2007·Journal of Clinical Microbiology·Hamid JalalChristopher Sonnex
Oct 26, 2007·Journal of Clinical Microbiology·Koen QuintUNKNOWN Costa Rican Vaccine Trial Group
Jan 5, 2008·Journal of Clinical Microbiology·Chung-Ter HuangShu-Ying Li
Apr 16, 2010·Journal of Clinical Microbiology·Matthew P StevensSepehr N Tabrizi
Mar 30, 2010·BMC Developmental Biology·Petra I zur Lage, Andrew P Jarman
Aug 23, 2005·Emerging Infectious Diseases·Servaas A MorréA Salvador Peña
Oct 5, 2010·Diagnostic Microbiology and Infectious Disease·Jian-Hong LiXiang-Sheng Chen
Mar 14, 2009·FEMS Immunology and Medical Microbiology·Lisbeth Nørum PedersenJens Kjølseth Møller
Jan 28, 2009·FEMS Immunology and Medical Microbiology·Servaas A MorréSander Ouburg
Nov 20, 2018·The Open Microbiology Journal·Valentina FeodorovaVladimir Motin
Feb 16, 2006·Journal of Medical Microbiology·Min-Chih HsuShu-Ying Li

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