Combination of resveratrol and 5-azacytydine improves osteogenesis of metabolic syndrome mesenchymal stem cells

Journal of Cellular and Molecular Medicine
Krzysztof MaryczChristine Weiss

Abstract

Endocrine disorders have become more and more frequently diagnosed in humans and animals. In horses, equine metabolic syndrome (EMS) is characterized by insulin resistance, hyperleptinemia, hyperinsulinemia, inflammation and usually by pathological obesity. Due to an increased inflammatory response in the adipose tissue, cytophysiological properties of adipose derived stem cells (ASC) have been impaired, which strongly limits their therapeutic potential. Excessive accumulation of reactive oxygen species, mitochondria deterioration and accelerated ageing of those cells affect their multipotency and restrict the effectiveness of the differentiation process. In the present study, we have treated ASC isolated from EMS individuals with a combination of 5-azacytydine (AZA) and resveratrol (RES) in order to reverse their aged phenotype and enhance osteogenic differentiation. Using SEM and confocal microscope, cell morphology, matrix mineralization and mitochondrial dynamics were assessed. Furthermore, we investigated the expression of osteogenic-related genes with RT-PCR. We also investigated the role of autophagy during differentiation and silenced PARKIN expression with siRNA. Obtained results indicated that AZA/RES significantly en...Continue Reading

References

Nov 18, 2005·Veterinary Immunology and Immunopathology·David J HurleyJames N Moore
Apr 14, 2010·Journal of Veterinary Internal Medicine·N FrankUNKNOWN American College of Veterinary Internal Medicine
May 7, 2010·Journal of Cell Science·Vandhana Muralidharan-ChariCrislyn D'Souza-Schorey
Jan 11, 2011·Human Molecular Genetics·Dena G HernandezAndrew B Singleton
Oct 15, 2011·PLoS Computational Biology·Jyh-Ying PengChun-Nan Hsu
Nov 18, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Barry A ChestnutLee J Martin
Jan 14, 2012·Veterinary Immunology and Immunopathology·M A de LaatC C Pollitt
May 17, 2012·International Reviews of Immunology·Urban Švajger, Matjaž Jeras
May 24, 2012·Diabetes·Antonello Pileggi
Mar 30, 2013·World Journal of Gastroenterology : WJG·Ke-Xin GanGuang-Yao Song
May 15, 2013·Archives of Medical Science : AMS·Marisa CoelhoRuben Fernandes
Feb 20, 2014·British Journal of Pharmacology·Pei-Wen ChengChing-Jiunn Tseng
Oct 26, 2014·Reviews in Endocrine & Metabolic Disorders·Nora Klöting, Matthias Blüher
Sep 1, 2013·Hormone Molecular Biology and Clinical Investigation·Guido Lastra, James R Sowers
Feb 3, 2015·Biochimica Et Biophysica Acta·Khushwant S Bhullar, Basil P Hubbard
May 1, 2015·PloS One·Shinsuke MatsuzakiTaiichi Katayama
Nov 17, 2015·Metabolic Syndrome and Related Disorders·Prasenjit Manna, Sushil K Jain
Sep 21, 2016·Stem Cells International·Sean D OwensDori L Borjesson
Jan 29, 2017·Redox Biology·Susana Rovira-LlopisVictor M Victor
Feb 27, 2017·Biochemical and Biophysical Research Communications·Ellen L RobbJeffrey A Stuart

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Citations

May 17, 2019·Journal of Clinical Medicine·Katarzyna KornickaKrzysztof Marycz
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Lin RenJuan Xia

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Methods Mentioned

BETA
ubiquitination
flow cytometry
ELISA
transmission electron microscopy
Assay
PCR
confocal microscopy

Software Mentioned

microP
GraphPad Prism
ImageJ
MitoRed
CellQuest Pro
Imaris

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