Abstract
To investigate the potential radiosensitization of S-1 and gefitinib in human non-small cell lung cancer (NSCLC) in vitro and in vivo. The impact of radiation, 5-fluorouracil (5-Fu), and gefitinib on the proliferation and apoptosis of human NSCLC A549, H1299, H1975, and HCC827 cells was examined by MTT and flow cytometry. The effect of radiation, 5-Fu, and gefitinib on the clonogenicity of H1975 and HCC827 cells was determined by colony formation assay. The effect of radiation, 5-fluorouracil (5-Fu), and gefitinib on the EGFR, AKT, and ERK1/2 activation in H1975 cells was determined by Western blot. The therapeutic efficacy of radiation, S-1, and gefitinib in the growth of implanted H1975 tumors and the AKT activation in the tumors were examined in vivo and immunohistochemistry, respectively. Combination of radiation, 5-Fu, and gefitinib significantly inhibited the proliferation of H1975 cells and triggered their apoptosis, but not other NSCLC cells tested. The combination therapy significantly mitigated the clonogenicity and attenuated the activation of EGFR and AKT signaling in H1975 cells. Furthermore, combination of S-1, gefitinib, and radiation significantly inhibited the growth of implanted H1975 tumors in mice and remark...Continue Reading
References
Feb 25, 2005·The New England Journal of Medicine·Susumu KobayashiBalázs Halmos
May 14, 2008·The Laryngoscope·Jay Patrick LopezWeg M Ongkeko
May 1, 2009·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Olga C J SchuurbiersJohan Bussink
Oct 23, 2010·Nature Reviews. Cancer·William Pao, Juliann Chmielecki
Jun 7, 2011·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yaping XuShenglin Ma
Jun 22, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mary Ellen UrickDeborah E Citrin
Jun 9, 2012·International Journal of Radiation Oncology, Biology, Physics·Masafumi IkedaTakuji Okusaka
May 23, 2013·International Journal of Oncology·Tarannum FerdousYoshiya Ueyama
Sep 4, 2013·The Annals of Thoracic Surgery·Masafumi YamaguchiYukito Ichinose
Dec 21, 2013·Annals of Oncology : Official Journal of the European Society for Medical Oncology·L S BlaszkowskyT S Hong
Mar 13, 2014·International Journal of Radiation Oncology, Biology, Physics·Sivan M BokobzaAnderson J Ryan
Aug 12, 2014·Expert Opinion on Pharmacotherapy·Masaaki Kawahara
Oct 29, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Puneeth IyengarRobert Timmerman
Nov 6, 2014·Oncology Reports·Min-Cong WangYu Yao
Jul 21, 2015·Seminars in Cancer Biology·Mahmoud Toulany, H Peter Rodemann
Nov 2, 2015·Clinical Lung Cancer·Anand SwaminathMark N Levine
Mar 24, 2016·BioMed Research International·Mao-hua ZhengKe-hu Yang
Jun 14, 2016·Journal of Thoracic Disease·Hosein RafiemaneshAli Moini
Nov 14, 2016·Cancer Letters·Amr S Abu LilaTatsuhiro Ishida
Jun 20, 2017·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Seiji NihoKoichi Goto
Aug 5, 2017·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y WangJ Sun