Combination of Salinomycin and AZD3463 Reveals Synergistic Effect on Reducing the Viability of T98G Glioblastoma Cells.

Anti-cancer Agents in Medicinal Chemistry
Aycan AsikCigir Biray Avci

Abstract

Salinomycin, an ionophore antibiotic, is known to be an effective agent in reducing the viability of Glioblastoma (GBM) cells. The combination of salinomycin with other chemotherapeutic drugs would help to overcome the drug resistance of GBM cells. This study aims to test the combinatorial effect of salinomycin and AZD3463 in T98G GBM cells. The cytotoxic effects of drugs on T98G GBM cells were determined by using WST-8 assay. Flow cytometry was used to identify apoptosis and cell cycle profiles after treatments. Real-time PCR was used to portray mRNA expression profiles of genes in the Wnt-signaling pathway after treatments. IC50 concentrations of AZD3463 and salinomycin were 529nM and 7.3μM for 48h, respectively. The combination concentrations of AZD3463 and salinomycin were 3.3μM and 333nM, respectively. The combination treatment showed a synergistic effect on reducing the viability of GBM cells. AZD3463, salinomycin, and their combination induced apoptosis in 1.2, 1.4, and 3.2 folds, respectively. AZD3463 and the combination treatment induced the cell cycle arrest at the G1 phase. Salinomycin and AZD3463 treatments, either alone or in combination, resulted in the downregulation or upregulation of mRNA expression levels of g...Continue Reading

References

Jul 22, 2009·Developmental Cell·Bryan T MacDonaldXi He
Oct 20, 2009·Biochemical and Biophysical Research Communications·Dominik FuchsCord Naujokat
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Desheng LuDennis A Carson
Dec 2, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tao MaRobert D Blitzer
Oct 2, 2012·Cold Spring Harbor Perspectives in Biology·Ken M Cadigan, Marian L Waterman
Aug 13, 2013·Current Medicinal Chemistry·Shuang ZhouErxi Wu
Jul 24, 2014·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Jigisha P ThakkarJohn L Villano
Mar 19, 2015·Frontiers in Oncology·Nicole Renee ParkerHelen Ruth Wheeler
Oct 20, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Fuat Kaplan, Fulya Teksen
Sep 13, 2016·Oncogene·T ZhanM Boutros
Dec 21, 2016·Journal of Clinical Pathology·George KaragkounisDina Tiniakos
Feb 2, 2017·The Journal of Clinical Investigation·Satoru Osuka, Erwin G Van Meir
Aug 3, 2017·International Journal of Oncology·Justin W Magrath, Yonghyun Kim
Sep 30, 2017·Cancer Medicine·Audelaure JuncaLucie Karayan-Tapon
Jun 19, 2018·Current Oncology·J M Rothenstein, N Chooback

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Oct 29, 2020·Biochemical and Biophysical Research Communications·Bakiye Goker BagcaCigir Biray Avci

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