Combination of survivin siRNA with neoadjuvant chemotherapy enhances apoptosis and reverses drug resistance in breast cancer MCF-7 cells

Journal of Cancer Research and Therapeutics
Honglin DongYonggang Lv

Abstract

Chemotherapeutic resistance is a main problem in clinical breast cancer therapy. The purpose of our study is to investigate whether the combination of neoadjuvant chemotherapy and survivin siRNA treatment could enhance the therapeutic effect of neoadjuvant chemotherapy using paclitaxel or epirubicin. The molecular cloning technique was applied to construct the expression vector of siRNA against survivin. Effectene Transfection Reagent was used to transfect plasmids to MCF-7 cells. Survivin expressions were detected by quantitative real-time PCR (qRT-PCR) and Western blot methods. The effect of paclitaxel or epirubicin, with or without the combination of survivin siRNA treatment, on drug susceptibility of MCF-7 cells was detected by CCK-8 assay. MCF-7 cell apoptosis was detected by Flow Cytometry. Survivin siRNA effectively inhibited the expression of Survivin RNA and protein levels (P < 0.05). Both paclitaxel and epirubicin can suppress the proliferation of MCF-7 cells and induce apoptosis to a certain degree respectively. The combination of survivin siRNA with the two chemotherapy drugs significantly enhanced both effects of the two chemotherapeutics respectively (P < 0.05). Survivin siRNA combined with the neoadjuvant chemoth...Continue Reading

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Citations

May 20, 2017·Drug Development and Industrial Pharmacy·Anish BabuRajagopal Ramesh
Jul 25, 2019·Revista da Associação Médica Brasileira·Glaucia Luciano da VeigaFernando Luiz Affonso Fonseca
Jan 2, 2021·Critical Reviews in Oncology/hematology·Shokooh SalimimoghadamBehzad Baradaran
Apr 23, 2021·Drug Design, Development and Therapy·Yifan WangChunying Cui

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