Combination of the electrogenic ionophores, valinomycin and CCCP, can lead to non-electrogenic K+/H+ exchange on bilayer lipid membranes

FEBS Letters
V N OrlovL S Yaguzhinsky

Abstract

The method of pH shift measuring by means of a pH microelectrode was applied to measure hydrogen ion fluxes across a planar bilayer lipid membrane (BLM) in the presence of the potassium ion ionophore, valinomycin, and a protonophore, carbonylcyanide m-chlorophenylhydrazone (CCCP), under conditions of the voltage clamp. The voltage dependence of the flux was determined to be in the range of +/- 150 mV under the conditions of both symmetrical KCl as well as a KCl gradient across the BLM. Surprisingly, at a clamped zero voltage on BLM a significant hydrogen ion flux was observed in the presence of a KCL gradient and both valinomycin and CCCP. This finding was interpreted as a result of induction of non-electrogenic K+/H+ exchange in the presence of valinomycin and CCCP, presumably through the formation of electrically neutral complexes of these two ionophores and K+ (H+) ions: valinomycin-K(+)-CCCP- and/or possibly valinomycin-CCCP(-)-H+.

References

Jan 1, 1976·Annual Review of Biochemistry·B C Pressman
Nov 1, 1992·Quarterly Reviews of Biophysics·S B Hladky
Jan 1, 1974·The Journal of Membrane Biology·M Foster, S McLaughlin
Jul 1, 1980·Physiological Reviews·S G McLaughlin, J P Dilger

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Citations

Jun 30, 2012·Journal of the American Chemical Society·Ye-Jin EunDouglas B Weibel
Apr 30, 2013·PloS One·Yuri N AntonenkoVladimir P Skulachev
Dec 21, 2016·Journal of Cellular Biochemistry·Mariángeles ClauzureTomás A Santa-Coloma
Apr 15, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Stephen J MoorePhilip A Gale

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