Combination of three miRNA (miR-141, miR-21, and miR-375) as potential diagnostic tool for prostate cancer recognition

International Urology and Nephrology
Paweł PorzyckiMirosław Tyrka

Abstract

Prostate cancer (PCa) is a common tumor disease in western countries and a leading cause of cancer-driven mortality in men. Current methods for prostate cancer detection, like prostate-specific antigen screening, lead to significant overtreatment. The purpose of the study was to analyze circulating microRNAs in serum as non-invasive biomarkers in patients with diagnosis of prostate cancer and healthy individuals. This preliminary study included a population of 20 patients with mean age of 68.6 years and mean PSA of 21.3 ng/ml. Eight healthy patients were used as control. MiRNAs were quantified in the total RNA fraction extracted from serum and levels of five microRNAs (miR-106b, miR-141, miR-21, mir-34a, and miR-375) were quantified by RT-qPCR. Statistical analyses evaluated correlation between clinicopathological data and miRNAs expression levels. Relative expression ratios of miR-106b, miR-141-3p, miR-21, and miR-375 were significantly increased (1.8-,  1.9-, 2.4-, and 2.6-fold, respectively) in the PCa group compared to healthy control. Using receiver operating characteristics, the highest area under the curve equal to 0.906 was obtained for miR-357 and indicates a very good diagnostic properties of this biomarker. We found ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 10, 2007·Cancer Research·Kati P PorkkaTapio Visakorpi
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Sep 1, 2009·Trends in Molecular Medicine·Paolo GandelliniNadia Zaffaroni
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Jan C BraseHolger Sültmann
Jan 29, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Fulya Yaman AgaogluUgur Gezer
Sep 13, 2011·PloS One·Michaela B KirschnerGlen Reid
Mar 24, 2012·Journal of Translational Medicine·Martina RedovaOndrej Slaby
May 2, 2012·BMC Bioinformatics·Chengxi YeDouglas W Yu
May 2, 2013·British Journal of Cancer·A Sita-LumsdenC L Bevan
Mar 26, 2014·Actas urologicas españolas·V Medina-VillaamilF Gómez-Veiga
Sep 16, 2014·International Journal of Cancer. Journal International Du Cancer·Jacques FerlayFreddie Bray
Mar 20, 2015·Drug Delivery and Translational Research·Christa HaldrupKarina D Sorensen
Apr 18, 2015·BMC Cancer·Silvia AntolínManuel Valladares-Ayerbes
Jul 15, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiaoying ZhouGuoxin Zhang
Aug 5, 2015·Journal of Clinical Medicine·Brian D KellyMichael J Kerin
Jan 12, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Xavier Filella, Laura Foj
Oct 30, 2016·International Journal of Molecular Sciences·Xavier Filella, Laura Foj
Mar 23, 2017·BMC Urology·Hung N LuuGiuliano Di Pietro
Aug 22, 2017·Cancer Letters·Rajnee KanwalSanjay Gupta
Sep 15, 2017·PloS One·Marilesia Ferreira de SouzaIlce Mara de Syllos Cólus
Jun 6, 2018·Cancer Biomarkers : Section a of Disease Markers·Yanying ZhaoKongxi Zhu

❮ Previous
Next ❯

Citations

Oct 3, 2019·Expert Review of Molecular Diagnostics·Emmy BoerrigterJack A Schalken
Feb 28, 2020·Medicina·Vlad Cristian MunteanuIoan Coman
Apr 9, 2020·Epigenomics·Irena AbramovicNino Sincic
May 14, 2020·Clinical and Experimental Medicine·Ilgiz GareevShiguang Zhao
Sep 15, 2020·Journal of Extracellular Vesicles·Marta CollettiAngela Di Giannatale
Jan 31, 2019·Diagnostics·Jochen Neuhaus, Bo Yang
Mar 19, 2019·Oncology Letters·Weiyun WuJuanhua Quan
Aug 30, 2019·Biomarkers in Medicine·Lynn Sorbara, Sudhir Srivastava
Dec 19, 2019·Frontiers in Oncology·Adil MalikJyotsna Batra
Aug 14, 2020·International Journal of Molecular Sciences·Ewa CiszkowiczMirosław Tyrka
Jan 25, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yudong WuDongming Xing
Mar 14, 2021·Biomarker Research·Ana E Jenike, Marc K Halushka
Mar 20, 2021·Therapeutic Advances in Urology·Anna SaltmanAbhinav Sidana
Aug 4, 2020·Asian Journal of Urology·Ieva EringyteClaire E Fletcher
Mar 22, 2021·Life Sciences·Yang LiuChaolai Man
Apr 4, 2021·Pharmaceutics·Noemi Arrighetti, Giovanni Luca Beretta
Sep 13, 2020·Pathology, Research and Practice·Cunjin NanYinghe Chen
Jun 27, 2021·Pharmacology & Therapeutics·Maria Adamaki, Vassilios Zoumpourlis
Jul 3, 2021·Cancers·Cora Palanca-BallesterAlfonso Calvo
Jul 8, 2021·Endocrinology·Megan LudwigJustin M Drake

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
reverse
PCR

Software Mentioned

GenEx
BLAST
Primer
STATISTICA

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.