Combination photoimmunotherapy with monoclonal antibodies recognizing different epitopes of human epidermal growth factor receptor 2: an assessment of phototherapeutic effect based on fluorescence molecular imaging

Oncotarget
Kimihiro ItoHisao Tajiri

Abstract

Photoimmunotherapy is a new class of molecular targeted cancer therapy based on a monoclonal antibody (mAb) conjugated to a photosensitizer and irradiation with near-infrared (NIR) light for both imaging and therapy. Here, we sought to determine the feasibility of combining photoimmunotherapy using conjugates of human epidermal growth factor receptor 2 (HER2)-specific mAb-photosensitizer IR700, trastuzumab-IR700 and pertuzumab-IR700. HER2-expressing and non-expressing cells were treated with mAb-IR700 conjugates and irradiated with NIR light. Fluorescence imaging and cytotoxic effects were examined in cultured HER2-expressng cancer cell lines and in a mouse tumor xenograft model. Trastuzumab-IR700 and pertuzumab-IR700 could specifically bind to HER2 without competing, and the combination treatment of both agents yielded stronger HER2-specific IR700 fluorescence signals than with the treatment with either agent singly. A cytotoxicity assay showed that the combination treatment of both trastuzumab-IR700 and pertuzumab-IR700 followed by NIR light irradiation induced stronger cytotoxic effect than with treatment of either agent plus NIR light irradiation. Furthermore, the phototoxic and cytotoxic effects of mAb depended on HER2-spe...Continue Reading

References

Jan 1, 1989·Cancer Chemotherapy and Pharmacology·M M Tomayko, C P Reynolds
Jul 10, 1987·Science·P P Di FioreS A Aaronson
Apr 12, 2000·Investigational New Drugs·J Nemunaitis
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
Apr 20, 2004·Cancer Cell·Matthew C FranklinMark X Sliwkowski
Oct 1, 2005·Nature Reviews. Cancer·Robert M Hoffman
Oct 21, 2005·The New England Journal of Medicine·Martine J Piccart-GebhartUNKNOWN Herceptin Adjuvant (HERA) Trial Study Team
Jun 8, 2006·Journal of Immunological Methods·Stephen P PerfettoMario Roederer
Jul 6, 2007·The New England Journal of Medicine·Clifford A Hudis
Feb 4, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·José BaselgaLuca Gianni
Jun 28, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yoriko Yamashita-KashimaKaori Fujimoto-Ouchi
Sep 11, 2012·Journal of Cellular Biochemistry·Masashi MomiyamaRobert M Hoffman
Jun 3, 2014·BMC Cancer·Takahito NakajimaHisataka Kobayashi
Nov 18, 2014·PloS One·Kazuhide SatoHisataka Kobayashi

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Citations

Nov 24, 2019·International Journal of Molecular Sciences·Haruka YamaguchiAndreas Evdokiou
Jun 29, 2018·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Yasuhiro OonoTomonori Yano
Nov 2, 2019·Cancer Medicine·Takashi NishimuraKoji Nakamura
Sep 10, 2020·Biomedicines·Fleury Augustin Nsole BitegheStefan Barth
Jul 16, 2019·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Takashi NishimuraMasayuki Saruta
Apr 25, 2020·Journal of Controlled Release : Official Journal of the Controlled Release Society·Marion M DekenSabrina Oliveira
Jun 28, 2019·Current Medicinal Chemistry·Zengtao WangShaoshun Li
Jun 3, 2021·Cancers·Takuya KatoHisataka Kobayashi
Aug 1, 2021·EBioMedicine·Yasuhiro MaruokaHisataka Kobayashi
Oct 26, 2021·Immunotherapy·Kohei MatsuokaKazuhide Sato

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Methods Mentioned

BETA
fluorescence imaging
fluorescence microscopy
Flow cytometry
xenograft
xenografts

Software Mentioned

ImageJ
GraphPad
GraphPad Prism

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