Combination therapy with human umbilical cord mesenchymal stem cells and angiotensin-converting enzyme 2 is superior for the treatment of acute lung ischemia-reperfusion injury in rats

Cell Biochemistry and Function
Xiaomiao ZhangZhenwei Liu

Abstract

Acute lung ischemia-reperfusion injury (ALIRI) is a serious disease that seriously affects human's life. In this study, we aimed to explore a more effective treatment method by combining human umbilical cord mesenchymal stem cells (HUMSCs) and angiotensin-converting enzyme 2 (ACE2) for ALIRI. Fifty rats were firstly divided into five groups, namely sham surgery group (sham) and four model groups (model, ACE2, HUMSCs and HUMSCs + ACE2) that were reperfused with 0.1 ml physiological saline (PS), 0.1 ml PS containing 1 × 10(6) lentiviral-ACE2/HUMSCs/ACE2 + UMSCs, respectively. Quantitative reverse transcription-PCR (qRT-PCR) and western blot assays were then conducted to detect the messenger RNA (mRNA) and protein levels of inflammatory cytokines [intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), tumour necrosis factor α (TNF-α), nuclear factor κB (NF-κB), platelet-derived growth factor (PDGF) and angiotensin II (Ang II)], antioxidant proteins [NAD(P)H quinone oxidoreductase 1 (NQO1), heme oxygenase 1 (HO-1)], DNA damage and apoptotic indicators [BCL2-associated X (Bax), cleaved caspase-3 (C-Csp 3), cleaved-poly(ADP-ribose) polymerase (C-PARP), Y-H2AX], anti-apoptotic indicator (Bcl-2) and smo...Continue Reading

References

Feb 5, 2000·American Journal of Respiratory Cell and Molecular Biology·K Kuwano, N Hara
May 4, 2000·The New England Journal of Medicine·L B Ware, M A Matthay
Aug 17, 2000·The New England Journal of Medicine
Apr 12, 2003·Critical Care Medicine·Thomas R MartinGustavo Matute-Bello
Nov 19, 2004·Cellular and Molecular Life Sciences : CMLS·F J WarnerA J Turner
Jul 8, 2005·Nature·Yumiko ImaiJosef M Penninger
Sep 21, 2005·American Journal of Respiratory Cell and Molecular Biology·Michael A Matthay, Guy A Zimmerman
Sep 22, 2005·Nature Reviews. Immunology·Michael Karin, Florian R Greten
Oct 21, 2005·The New England Journal of Medicine·Gordon D RubenfeldLeonard D Hudson
Sep 23, 2006·The New England Journal of Medicine·Ketil LundeKolbjørn Forfang
Sep 23, 2006·The New England Journal of Medicine·Volker SchächingerUNKNOWN REPAIR-AMI Investigators
Mar 17, 2007·Transplantation Proceedings·H B BittnerF W Mohr
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naveen GuptaMichael A Matthay
Nov 21, 2007·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Phil BothaSteven C Clark
Mar 5, 2009·The Journal of Thoracic and Cardiovascular Surgery·Gorav AilawadiDavid R Jones
Aug 14, 2010·Current Drug Targets·Chintan M Raval, Patty J Lee
Aug 31, 2010·Translational Research : the Journal of Laboratory and Clinical Medicine·Viranuj Sueblinvong, Daniel J Weiss
Jun 16, 2012·Journal of Cellular and Molecular Medicine·Zhao-hui QinLong Yu
Aug 2, 2014·Environmental Toxicology·Yi-Chun HuangYu-Hsiang Kuan
Jul 1, 2006·Drug Discovery Today. Disease Mechanisms·Yumiko ImaiJosef M Penninger

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