Combination treatment with irritant and recombinant interleukin 2 in the peritoneal cavity for evoking effective anti-tumor activity: generation of lymphokine-activated killer cells and tumor-specific killer cells

Japanese Journal of Cancer Research : Gann
H UchidaK Nomoto

Abstract

Meth A sarcoma, growing in the subcutaneous tissue of syngeneic BALB/c mice, regressed completely after an intraperitoneal (ip) injection of proteose peptone (PP) (on day 6) followed by 2 ip administrations (on days 7 and 8) of human recombinant interleukin-2 (IL-2, 25 micrograms/day), whereas one such treatment alone had little effect on the tumor growth. While this combination treatment was effective in anti-asialo GM1 antibody-treated mice, no such effect was noted in T cell-depleted ATXFL (thymectomized, irradiated and fetal liver cell-reconstituted) mice. These results show that T cells are mainly responsible for this antitumor effect. Treatment with a combination of PP and IL-2, but not with either PP or IL-2 alone, resulted in a marked increase in the T cell population in the peritoneal cavity after the treatment. At an early stage after the combination treatment, both peritoneal exudate cells (PEC) and spleen cells exhibited killing activity with a promiscuous specificity. However, at a later stage, 7 days after the treatment, Meth A-specific killer activity was observed in both PEC and the spleen. Meth A rechallenge was rejected by the mice in which the tumor had regressed, but the antigenically different Meth 1 was ac...Continue Reading

References

Apr 16, 1985·Biochemical and Biophysical Research Communications·K NaruoK Tsukamoto
Jul 31, 1985·Biochemical and Biophysical Research Communications·K KatoA Kakinuma
Mar 15, 1972·International Journal of Cancer. Journal International Du Cancer·H O SjögrenK E Hellström
Mar 15, 1971·International Journal of Cancer. Journal International Du Cancer·I HellströmK E Hellström
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·A Uchida, M Micksche
Feb 1, 1961·The Journal of Immunology : Official Journal of the American Association of Immunologists·H J WINN

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