Combinations of Ashwagandha leaf extracts protect brain-derived cells against oxidative stress and induce differentiation

PloS One
Navjot ShahR Wadhwa

Abstract

Ashwagandha, a traditional Indian herb, has been known for its variety of therapeutic activities. We earlier demonstrated anticancer activities in the alcoholic and water extracts of the leaves that were mediated by activation of tumor suppressor functions and oxidative stress in cancer cells. Low doses of these extracts were shown to possess neuroprotective activities in vitro and in vivo assays. We used cultured glioblastoma and neuroblastoma cells to examine the effect of extracts (alcoholic and water) as well as their bioactive components for neuroprotective activities against oxidative stress. Various biochemical and imaging assays on the marker proteins of glial and neuronal cells were performed along with their survival profiles in control, stressed and recovered conditions. We found that the extracts and one of the purified components, withanone, when used at a low dose, protected the glial and neuronal cells from oxidative as well as glutamate insult, and induced their differentiation per se. Furthermore, the combinations of extracts and active component were highly potent endorsing the therapeutic merit of the combinational approach. Ashwagandha leaf derived bioactive compounds have neuroprotective potential and may s...Continue Reading

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Citations

Sep 13, 2015·Neurochemistry International·Renu WadhwaSunil C Kaul
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May 22, 2019·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Sukant GargRenu Wadhwa
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Dec 23, 2016·Molecular and Cellular Biochemistry·Taranjeet KaurGurcharan Kaur
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Jul 1, 2021·Virusdisease·Sonali Bhattacharya, Sudipta Majumdar Nee Paul

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Methods Mentioned

BETA
nuclear translocation

Software Mentioned

AxioVision
Imaris
GraphPad

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