Combinations of fluorescently labeled pulmonary surfactant proteins SP-B and SP-C in phospholipid films

Biophysical Journal
K NagK M Keough

Abstract

Hydrophobic pulmonary surfactant (PS) proteins B (SP-B) and C (SP-C) modulate the surface properties of PS lipids. Epifluorescence microscopy was performed on solvent-spread monolayers of fluorescently labeled porcine SP-B (R-SP-B, labeled with Texas Red) and SP-C (F-SP-C, labeled with fluorescein) in dipalmitoylphosphatidylcholine (DPPC) (at protein concentrations of 10 and 20 wt%, and 10 wt% of both) under conditions of cyclic compression and expansion. Matrix-assisted laser desorption/ionization (MALDI) spectroscopy of R-SP-B and F-SP-C indicated that the proteins were intact and labeled with the appropriate fluorescent probe. The monolayers were compressed and expanded for four cycles at an initial rate of 0.64 A2 x mol(-1) x s(-1) (333 mm2 x s x [-1]) up to a surface pressure pi approximately 65 mN/m, and pi-area per residue (pi-A) isotherms at 22 +/- 1 degrees C were obtained. The monolayers were microscopically observed for the fluorescence emission of the individual proteins present in the film lipid matrix, and their visual features were video recorded for image analysis. The pi-A isotherms of the DPPC/protein monolayers showed characteristic "squeeze out" effects at pi approximately 43 mN/m for R-SP-B and 55 mN/m for ...Continue Reading

Citations

Aug 25, 2001·Analytical Biochemistry·I PlasenciaJ Pérez-Gil
Nov 4, 2008·European Biophysics Journal : EBJ·Albena JordanovaZdravko Lalchev
Dec 19, 2002·Biophysical Chemistry·Bohdana M DischerStephen B Hall
Nov 14, 1998·Biochimica Et Biophysica Acta·R VeldhuizenF Possmayer
May 23, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·F PossmayerS Schürch
Jun 21, 2012·The European Physical Journal. E, Soft Matter·F CampeloA Hernández-Machado
Nov 25, 2003·American Journal of Respiratory Cell and Molecular Biology·Amiya K PandaFred Possmayer
Sep 15, 2014·European Journal of Medicinal Chemistry·Ramona GalatolaIsabel Haro
Sep 22, 2012·European Biophysics Journal : EBJ·Bárbara OlmedaJesús Pérez-Gil
Oct 14, 2014·Biochimica Et Biophysica Acta·Nuria RoldanBegoña Garcia-Alvarez
Jun 5, 2012·Biochimica Et Biophysica Acta·Cristina Casals, Olga Cañadas
Nov 30, 2010·Biochimica Et Biophysica Acta·Eleonora KeatingNils O Petersen
Mar 1, 2016·Biochimica Et Biophysica Acta·Svetlana Baoukina, D Peter Tieleman
Jul 20, 2011·Biochimica Et Biophysica Acta·Susan L DuncanRonald G Larson
Jan 10, 2012·Biophysical Journal·Prajnaparamita DharJoseph A Zasadzinski
Nov 3, 2009·Biochimica Et Biophysica Acta·Mohammed Saleem, Hans-Joachim Galla
Dec 7, 2000·Biophysical Journal·E J VeldhuizenH P Haagsman
Sep 28, 2014·Chemistry and Physics of Lipids·Elisa Parra, Jesús Pérez-Gil
Apr 8, 2006·Chemistry and Physics of Lipids·Alicia G Serrano, Jesús Pérez-Gil
Jan 5, 2006·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·S ParmigianiG Bevilacqua
Oct 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Roberta Bruni
Dec 22, 1999·The American Journal of Physiology·K NagF Possmayer
Mar 24, 2021·Archives of Biochemistry and Biophysics·José Carlos Castillo-SánchezJesús Pérez-Gil

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