Abstract
High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for the expression of resistance. In this work, we determined whether specific changes in PBPs were associated with specific MurM variants. Two collections of highly penicillin-resistant (MIC 2-8 mg/L) isolates, including 10 early (1997-1998) and 23 contemporary (2002-2004) isolates, were studied. Most of the isolates belonged to clones Spain(6B)-2 (13 strains), Spain(23F)-1 (10 isolates) and Spain(14)-5 (20 isolates). Different protein variants of MurM (MA, MB5, MB6, MB9 and MB10), PBP1A (A-C), PBP2B (A-D) and PBP2X (A-C) were recognized, including two murM alleles not previously described. Particular [MurM-PBP1A-2B-2X] allelic combinations were predominant among the different clones, including [MA-B-B-B] for old (MIC 2 mg/L) and [MB10-C-A-B] for recent (MIC 4-8 mg/L) Spain(6B)-2 isolates, [MA-A-C-A] for Spain(23F)-1 and [MB5-A-A-A] in Spain(14)-5 isolates. Although S. pneumoniae has a basic recombinational population structure, our results indicate remarkable conservation of PBPs and MurM protein types within each clone. This suggests that particular P...Continue Reading
References
Aug 1, 1991·The Journal of Infectious Diseases·R MuñozB G Spratt
Jan 1, 1989·Molecular Microbiology·C G DowsonB G Spratt
Nov 18, 1998·Journal of Clinical Microbiology·A FenollJ Casal
Nov 14, 2000·Journal of Bacteriology·S R FilipeA Tomasz
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J VerhaegenL Verbist
Jul 14, 2001·Antimicrobial Agents and Chemotherapy·A M Smith, K P Klugman
Aug 15, 2001·Antimicrobial Agents and Chemotherapy·A M SmithK P Klugman
Feb 7, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sergio R FilipeAlexander Tomasz
Jul 18, 2002·Antimicrobial Agents and Chemotherapy·Mignon du PlessisKeith P Klugman
Apr 28, 2005·Antimicrobial Agents and Chemotherapy·Anthony M SmithKeith P Klugman
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Laurent ChesnelAndré Zapun
Dec 22, 2005·The Journal of Antimicrobial Chemotherapy·Fabio CafiniUNKNOWN Spanish Pneumococcal Network (G03/103)
Citations
Sep 19, 2008·The Journal of Antimicrobial Chemotherapy·Francisco SorianoAsuncion Fenoll
Mar 3, 2010·Antimicrobial Agents and Chemotherapy·K Kosowska-ShickP C Appelbaum
Mar 3, 2010·Antimicrobial Agents and Chemotherapy·Ewa SadowyWaleria Hryniewicz
Mar 26, 2008·Antimicrobial Agents and Chemotherapy·Elia Gómez G de la PedrosaRafael Cantón
Mar 25, 2009·Antimicrobial Agents and Chemotherapy·Klaudia Kosowska-ShickPeter C Appelbaum
Feb 25, 2009·Antimicrobial Agents and Chemotherapy·Klaudia Kosowska-ShickPeter C Appelbaum
Jan 15, 2008·Annals of Clinical Microbiology and Antimicrobials·Stephen G JenkinsDavid J Farrell
Feb 22, 2012·Microbes and Infection·J E Cornick, S D Bentley
Apr 20, 2010·Enfermedades infecciosas y microbiología clínica·Carmen Torres, Emilia Cercenado
May 4, 2007·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Michael J StanhopeHeather Amrine-Madsen
May 27, 2015·Molecular Microbiology·Katya TodorovaRegine Hakenbeck
Apr 25, 2012·International Journal of Antimicrobial Agents·Todd A DaviesA Simon Lynch
Jun 29, 2007·The Journal of Antimicrobial Chemotherapy·Raffaella KoncanRosa del Campo