Combinatorial Activity of Flavonoids with Antibiotics Against Drug-Resistant Staphylococcus aureus

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Ana Cristina AbreuManuel Simões

Abstract

The use of resistance-modifying agents is a potential strategy that is used to prolong the effective life of antibiotics in the face of increasing antibiotic resistance. Since certain flavonoids are potent bacterial efflux pump inhibitors, we assessed morin, rutin, quercetin, hesperidin, and (+)-catechin for their combined activity with the antibiotics ciprofloxacin, tetracycline, erythromycin, oxacillin, and ampicillin against drug-resistant strains of Staphylococcus aureus, including methicillin-resistant S. aureus. Four established methods were used to determine the combined efficacy of each combination: microdilution checkerboard assays, time-kill determinations, the Etest, and dual disc-diffusion methods. The cytotoxicity of the flavonoids was additionally evaluated in a mouse fibroblast cell line. Quercetin and its isomer morin decreased by 3- to 16-fold the minimal inhibitory concentration of ciprofloxacin, tetracycline, and erythromycin against some S. aureus strains. Rutin, hesperidin, and (+)-catechin did not promote any potentiation of antibiotics. Despite the potential cytotoxicity of these phytochemicals at a high concentration (fibroblast IC50 of 41.8 and 67.5 mg/L, respectively), quercetin is commonly used as a s...Continue Reading

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Citations

Jul 12, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anabela BorgesManuel Simões
Jun 30, 2019·Critical Reviews in Microbiology·Rene Christena LowrenceSaisubramanian Nagarajan
Mar 25, 2017·Future Microbiology·Menachem Shoham, Michael Greenberg
Dec 15, 2020·Journal of Biomolecular Structure & Dynamics·Balajee RamachandranShyi-Long Lee
Mar 9, 2021·Journal of Bioenergetics and Biomembranes·Joycy F S Dos SantosFrancisco A B Cunha
Mar 26, 2019·Journal of Agricultural and Food Chemistry·Christopher S ChambersKateřina Valentová

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Methods Mentioned

BETA
Assay

Software Mentioned

QUERC
MOR
Etest
SPSS

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