Combinatorial anti-proliferative effects of tamoxifen and naringenin: The role of four estrogen receptor subtypes

Toxicology
Zhixiang XuXuejun Pan

Abstract

Breast cancer is the most diagnosed diseases and the second-leading cause of death in females, among which the estrogen receptor positive (ER+) patients are more common of all cases. In present study, we selected MCF-7 as an in vitro model and investigated the combinatorial anti-proliferative effects of tamoxifen and naringenin on ER+ breast cancer, and then explored the potential mechanisms involved in mitochondrial dysfunction and oxidative stress mediated by several estrogen receptor subtypes. Six assessment endpoints including cell viability, cell migration, cell cycle, cell apoptosis, mRNA, and protein expression were estimated. Tamoxifen and naringenin were shown to inhibit the cell growth of MCF-7 cells at higher concentrations, and co-exposure with them significantly inhibited cell proliferation in an additive manner. Results from a wound healing assay indicated that the combined treatment of tamoxifen and naringenin markedly suppressed cell migration compared with the single exposure by downregulating the expression of MMP-9 and MMP-2. Flow cytometry analysis revealed that the combined treatment of tamoxifen and naringenin blocked cell cycle in G2/M phase through suppressing the transcription of cell cycle regulation p...Continue Reading

Citations

Nov 24, 2018·Environmental Science and Pollution Research International·Bin HuangXuejun Pan
May 31, 2019·International Journal of Molecular Sciences·Charlène ThiebautHélène Dumond
Feb 20, 2021·Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society·Adam HermawanHaruma Anggraini Muflikhasari
May 3, 2021·Regulatory Toxicology and Pharmacology : RTP·Yonggang WangPeibo Li
Nov 10, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Zahra MemarianiAroona Chabra
Nov 6, 2021·Oncology Reports·Bushra ZeyaM Moshahid A Rizvi

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