Combinatorial antibody libraries from survivors of the Turkish H5N1 avian influenza outbreak reveal virus neutralization strategies.

Proceedings of the National Academy of Sciences of the United States of America
Arun K KashyapRichard A Lerner

Abstract

The widespread incidence of H5N1 influenza viruses in bird populations poses risks to human health. Although the virus has not yet adapted for facile transmission between humans, it can cause severe disease and often death. Here we report the generation of combinatorial antibody libraries from the bone marrow of five survivors of the recent H5N1 avian influenza outbreak in Turkey. To date, these libraries have yielded >300 unique antibodies against H5N1 viral antigens. Among these antibodies, we have identified several broadly reactive neutralizing antibodies that could be used for passive immunization against H5N1 virus or as guides for vaccine design. The large number of antibodies obtained from these survivors provide a detailed immunochemical analysis of individual human solutions to virus neutralization in the setting of an actual virulent influenza outbreak. Remarkably, three of these antibodies neutralized both H1 and H5 subtype influenza viruses.

References

Mar 20, 1992·Journal of Molecular Biology·J Foote, G Winter
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A S KangD R Burton
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·U KalinkeH Hengartner
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M A HolmesJ Foote
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chih-chin HuangPeter D Kwong
Nov 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Brunhilde Felding-HabermannKim D Janda
Jun 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Arvind RajpalRoberto Crea
Aug 31, 2006·Annals of Internal Medicine·Thomas C LukeStephen L Hoffman
Nov 25, 2006·The New England Journal of Medicine·Ahmet Faik OnerMehmet Ceyhan
Oct 5, 2007·The New England Journal of Medicine·Boping ZhouYi Guan

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Citations

Jun 6, 2013·Virus Research·Guus F Rimmelzwaan, Jacqueline M Katz
Apr 30, 2010·Expert Reviews in Molecular Medicine·Neetu SinghSuresh K Mittal
May 9, 2012·Nature Biotechnology·Barton F HaynesThomas B Kepler
May 19, 2009·Nature Immunology·Peter D Kwong, Ian A Wilson
Apr 8, 2009·Nature Medicine·Kirsten Dorans
Nov 7, 2009·Nature Medicine·Grace L Chen, Kanta Subbarao
Sep 26, 2012·Nature Reviews. Immunology·Patrick C Wilson, Sarah F Andrews
Feb 24, 2009·Nature Structural & Molecular Biology·Jianhua SuiWayne A Marasco
Feb 24, 2009·Nature Structural & Molecular Biology·Taia T Wang, Peter Palese
Feb 12, 2013·Nature Structural & Molecular Biology·Rui XuIan A Wilson
Jul 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Li XuRichard A Lerner
Oct 14, 2009·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Chi WangChi-Huey Wong
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Natalie PicaPeter Palese
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Peter S LeeIan A Wilson
Apr 5, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jianhua SuiWayne A Marasco
Apr 1, 2011·The Journal of Infectious Diseases·Lorena Itatí IbañezXavier Saelens
Oct 23, 2012·The Journal of Infectious Diseases·Matthew S MillerPeter Palese
Mar 3, 2009·Science·Damian C EkiertIan A Wilson
Aug 28, 2010·Science·Robert W Doms
Jul 9, 2011·Science·Damian C EkiertJaap Goudsmit
Aug 13, 2011·Science·Taia T Wang, Peter Palese
Jan 12, 2010·Annual Review of Medicine·Suryaprakash Sambhara, Gregory A Poland
Apr 15, 2010·The Journal of Clinical Investigation·Davide CortiAntonio Lanzavecchia
Apr 22, 2009·PLoS Medicine·Hui-Ling Yen, J S Malik Peiris
Jun 26, 2009·PLoS Medicine·Nicholas J WhiteTimothy M Uyeki
Sep 14, 2011·PLoS Neglected Tropical Diseases·Adly M M Abd-AllaMax Bergoin

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