Combinatorial-approached neuroprotection using pan-caspase inhibitor and poly (ADP-ribose) polymerase (PARP) inhibitor following experimental stroke in rats; is there additional benefit?

Brain Research
Elgin YapYee-Kong Ng

Abstract

Energy requiring apoptosis and presumably unregulated necrosis are considered conceptually and morphologically distinct forms of cell death which have been initially identified as two exclusive pathways. However, several apoptotic characteristics have been observed in the necrotic core lesion in ischemia which led to the controversial theory that cell death advances via a number of hybrid pathways among a continuum between the two processes. ATP availability has been shown to influence the decision between apoptosis and necrosis. The aims of our study are 1) to determine if combined inhibitors administration of pan-caspase inhibitor Carbobenzoxy-Val-Ala-Asp-fluoromethylketone (z-VAD-fmk) and non-selective poly (ADP-ribose) polymerase (PARP) inhibitor 3-aminobenzamide (3-AB) can further reduce infarct volume compared to single modality of either inhibitor following ischemic insult, 2) to ascertain the pharmacological intervention up to 24 hour post-middle cerebral artery occlusion (MCAo), and 3) to correlate intracellular ATP level with infarct volume. Single modality treatment was optimised at 3 mg/kg z-VAD-fmk and 30 mg/kg 3-AB with infarct volume measured at 24.13%+/-3.89% and 26.98%+/-2.22% respectively, while untreated cont...Continue Reading

References

Mar 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R A SwansonF R Sharp
Jan 1, 1989·Stroke; a Journal of Cerebral Circulation·E Z LongaR Cummins
Oct 1, 1996·Current Opinion in Neurobiology·D W Choi
Aug 1, 1997·Trends in Biochemical Sciences·D W Nicholson, N A Thornberry
Mar 14, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·M EndresM A Moskowitz
Jun 6, 1998·The Journal of Experimental Medicine·D VercammenP Vandenabeele
Mar 17, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A N MurphyM F Beal
May 7, 1999·Stroke; a Journal of Cerebral Circulation·J VogelW Kuschinsky
Jun 12, 1999·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·J AronowskiJ C Grotta
Sep 11, 1999·Trends in Neurosciences·M Roy, R Sapolsky
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·H C Ha, S H Snyder
Apr 20, 2001·Brain Research. Molecular Brain Research·D C HarrisonK L Philpott
Mar 7, 2002·Trends in Pharmacological Sciences·Alberto Chiarugi
Sep 2, 2003·Journal of Neuroscience Research·Natalia V GulyaevaIrina V Kudryashova
Jan 28, 2004·Cell·Nika N Danial, Stanley J Korsmeyer
Jul 30, 2004·Biochimica Et Biophysica Acta·Denis S IzyumovVladimir P Skulachev

❮ Previous
Next ❯

Citations

Jan 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Junying Yuan
Apr 23, 2010·Molecular Neurobiology·Robert Piotr StrosznajderJoanna Benigna Strosznajder
Dec 11, 2013·Translational Stroke Research·Mingke Song, Shan Ping Yu
Sep 28, 2012·International Journal of Stroke : Official Journal of the International Stroke Society·Neetu SinghKashif Hanif
Apr 2, 2014·British Journal of Pharmacology·Amos A FatokunTed M Dawson
Jul 17, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Mingjuan SunQifeng Yang
Nov 26, 2013·Biochemical and Biophysical Research Communications·Cong QiuJun Yu
May 25, 2021·Frontiers in Neurology·Raymond C KoehlerTed M Dawson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.