Combinatorial engineering of hybrid mevalonate pathways in Escherichia coli for protoilludene production

Microbial Cell Factories
Liyang YangSeon-Won Kim

Abstract

Protoilludene is a valuable sesquiterpene and serves as a precursor for several medicinal compounds and antimicrobial chemicals. It can be synthesized by heterologous expression of protoilludene synthase in Escherichia coli with overexpression of mevalonate (MVA) or methylerythritol-phosphate (MEP) pathway, and farnesyl diphosphate (FPP) synthase. Here, we present E. coli as a cell factory for protoilludene production. Protoilludene was successfully produced in E. coli by overexpression of a hybrid exogenous MVA pathway, endogenous FPP synthase (IspA), and protoilludene synthase (OMP7) of Omphalotus olearius. For improving protoilludene production, the MVA pathway was engineered to increase synthesis of building blocks isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) by sequential order permutation of the lower MVA portion (MvL), the alteration of promoters and copy numbers for the upper MVA portion (MvU), and the coordination of both portions, resulting in an efficient entire MVA pathway. To reduce the accumulation of mevalonate observed in the culture broth due to lower efficiency of the MvL than the MvU, the MvL was further engineered by homolog substitution with the corresponding genes from Staphylococcus...Continue Reading

References

Jun 5, 2003·Nature Biotechnology·Vincent J J MartinJay D Keasling
Feb 5, 2004·Phytochemistry·Matías ReinaBraulio M Fraga
Dec 29, 2006·The Journal of Antibiotics·Xing-Na WangJi-Kai Liu
Dec 30, 2006·Applied and Environmental Microbiology·Tomoko NishizakiHiroshi Yanagawa
Mar 9, 2010·Journal of Biological Engineering·J Christopher AndersonJay D Keasling
Dec 25, 2010·Current Medicinal Chemistry·R SchobertB Biersack
Jun 15, 2011·Proceedings of the National Academy of Sciences of the United States of America·Han N LimRazika Hussein
Sep 13, 2011·Metabolic Engineering·Chonglong WangSeon-Won Kim
Nov 22, 2013·Applied Microbiology and Biotechnology·Farnaz F NowrooziJay D Keasling

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Citations

Oct 11, 2016·Microbial Biotechnology·Lorena Jiménez-DíazAna Segura
Jul 22, 2020·Biotechnology and Bioengineering·Yunhe MengChonglong Wang
Jan 5, 2021·Applied Microbiology and Biotechnology·Govinda R NavaleSandip S Shinde
May 29, 2020·Metabolic Engineering·Quentin M DudleyMichael C Jewett
May 18, 2021·The New Phytologist·Constantine GaragounisKalliope K Papadopoulou
Jul 15, 2021·Critical Reviews in Biotechnology·Chun-Li LiuTianwei Tan
Jul 8, 2021·Natural Product Reports·Mauro A RinaldiNigel S Scrutton
Aug 12, 2021·Journal of Agricultural and Food Chemistry·Jie MaiXiao-Jun Ji

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Datasets Mentioned

BETA
AE007317.1

Methods Mentioned

BETA
PCR

Software Mentioned

BglBricks
pSNA

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