Combinatorial in Vitro and in Silico Approach To Describe Shear-Force Dependent Uptake of Nanoparticles in Microfluidic Vascular Models

Analytical Chemistry
Verena CharwatPeter Ertl

Abstract

In the present work, we combine experimental and computational methods to define the critical shear stress as an alternative parameter for nanotoxicological and nanomedical evaluations using an in vitro microfluidic vascular model. We demonstrate that our complementary in vitro and in silico approach is well suited to assess the fluid flow velocity above which clathrin-mediated (active) nanoparticle uptake per cell decreases drastically although higher numbers of nanoparticles per cell are introduced. Results of our study revealed a critical shear stress of 1.8 dyn/cm2, where maximum active cellular nanoparticle uptake took place, followed by a 70% decrease in uptake of 249 nm nanoparticles at 10 dyn/cm2, respectively. The observed nonlinear relationship between flow velocity and nanoparticle uptake strongly suggests that fluid mechanical forces also need to be considered in order to predict potential in vivo distribution, bioaccumulation, and clearance of nanomaterials and novel nanodrugs.

References

Jan 1, 1977·Annual Review of Biochemistry·S C SilversteinZ A Cohn
Apr 1, 1989·Cell Biophysics·D A Hammer, D A Lauffenburger
Nov 1, 1995·Journal of Magnetic Resonance Imaging : JMRI·J N OshinskiR I Pettigrew
Mar 1, 1997·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·S OyreW P Paaske
Feb 11, 2003·Vascular and Endovascular Surgery·Jacek J Paszkowiak, Alan Dardik
Aug 9, 2008·International Journal of Nanomedicine·Wim H De Jong, Paul J A Borm
Nov 26, 2008·Nature Clinical Practice. Cardiovascular Medicine·Peter F Davies
Oct 28, 2009·Journal of Visualized Experiments : JoVE·Carolyn G ConantCristian Ionescu-Zanetti
Jun 21, 2011·Lab on a Chip·Lukas RichterPeter Ertl
Nov 1, 2011·Annual Review of Pharmacology and Toxicology·S M MoghimiT L Andresen
Feb 26, 2013·PloS One·Jessie S JeonJoseph L Charest
Mar 2, 2013·Nature Reviews. Drug Discovery·Rudy Juliano
Apr 13, 2013·Current Opinion in Biotechnology·Larissa Y RizzoTwan Lammers
Dec 18, 2013·Microvascular Research·Hyunjae LeeNoo Li Jeon
Feb 5, 2014·Journal of Nanobiotechnology·Li ShangGerd Ulrich Nienhaus
Feb 27, 2014·Analytical Chemistry·Zeynep Dereli-KorkutSihong Wang
Apr 15, 2014·Trends in Biotechnology·Peter ErtlGünter Lepperdinger
Jul 23, 2014·Nanotoxicology·Sanjeev Kumar MahtoJosué Sznitman
May 26, 2015·Biomedical Microdevices·Ruiqing HuNaoki Ono
Jul 3, 2015·Biotechnology Advances·Mario RothbauerPeter Ertl
Nov 17, 2017·Lab on a Chip·Mario RothbauerPeter Ertl

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