Combinatorial materials research applied to the development of new surface coatings I: a multiwell plate screening method for the high-throughput assessment of bacterial biofilm retention on surfaces

Journal of Combinatorial Chemistry
Shane J StafslienPhilip Boudjouk

Abstract

Combinatorial, high-throughput capabilities have been established to aid in the rapid development of new and effective antifouling marine coatings for naval applications. A biological screening process involving marine bacteria was developed that allows for rapid and effective quantification of bacterial biofilm growth and retention on large numbers of coating surfaces in parallel. The screening process involves (1) multiwell plate modifications for coating deposition, (2) deposition of combinatorial coating libraries via an automated liquid dispensing robot, (3) coating thickness measurements of cured coatings, (4) preconditioning of coatings via immersion in deionized water, (5) bacterial incubation, (6) plate processing, and (7) data analysis for identification of promising candidates. The details of the method developed are described in this document.

References

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Citations

Jun 19, 2010·Archives of Microbiology·Birgit M PrüssAnne Denton
Jun 8, 2011·ACS Combinatorial Science·Radislav PotyrailoHubert Lam
Jul 27, 2007·Biofouling·Dean C WebsterShane J Stafslien
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Dec 7, 2007·Biofouling·D RittschofE Holm
Nov 21, 2009·Biofouling·Stephanie E M ThompsonJames A Callow
Jul 29, 2010·Journal of Biomedical Materials Research. Part a·Zhigang ChenSandeep Patel
Apr 8, 2016·BMC Microbiology·Katrin LeinweberPeter G Kroth
Oct 5, 2016·ACS Applied Materials & Interfaces·Teluka P GalhenageDean C Webster
Nov 26, 2009·International nursing review·F A Yildiz, M N Esin
May 3, 2013·PloS One·Iztok DogsaInes Mandic-Mulec
Feb 16, 2013·Microbiology·Warren L SimmonsKevin Dybvig
Nov 7, 2017·Polymer Chemistry·Melissa L HawkinsMelissa A Grunlan
May 12, 2018·International Journal of Molecular Sciences·Maria SaltaJulian A Wharton
Aug 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Shuji YeZhan Chen
Jan 24, 2008·Chemical Reviews·Radislav A Potyrailo, Vladimir M Mirsky

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