Combined amino acid mutations occurring in the envelope closely correlate with pathogenicity of EIAV

Archives of Virology
Hua LiangY M Shao

Abstract

The Chinese equine infectious anemia virus (EIAV) donkey-leukocyte attenuated vaccine (DLV) provides a unique natural model system to study the attenuation mechanism and immunological control of lentivirus replication. Critical consensus mutations were identified between virulent Chinese EIAV strains and vaccine strains. Based on a full-length infectious clone of EIAV vaccine strain pLGFD3, two molecular clones, mFD5-4-7 and mFD7-2-11, were successfully constructed, in which 4 and 6 critical consensus mutations in the env gene of the vaccine strain were point-mutated to the wild-type sequence, respectively by an overlap PCR mutagenesis strategy. The infectivity, virulence, and pathogenesis of the constructed clones were investigated in vitro using a reverse transcriptase assay, an indirect immunofluorescence assay, observation of cytopathogenic effect, and virion observation as well as in vivo by inoculation of animals with the resulting infectious clones. The pathogenic symptoms in horses inoculated with mFD7-2-11 were more severe than those inoculated with mFD5-4-7, whereas no pathogenic symptoms were detected in animals inoculated with their parental clone pLGFD3 strain. The results indicate that the consensus mutation resid...Continue Reading

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Citations

Nov 5, 2013·Veterinary Microbiology·R F CookC J Issel
Apr 21, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Pei Long SunXin Qi Liu
Sep 27, 2014·Applied Microbiology and Biotechnology·Zhe HuXiaojun Wang
Dec 3, 2014·The Veterinary Clinics of North America. Equine Practice·Charles J IsselDavid W Horohov
Feb 5, 2016·Retrovirology·Xue-Feng WangJian-Hua Zhou
Feb 26, 2008·Virus Research·Qinglai MengYiming Shao
Feb 9, 2018·Oncotarget·Hua-Nan WangJian-Guo Dong

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