Combined Application of Bacterial Predation and Violacein to Kill Polymicrobial Pathogenic Communities

Scientific Reports
Hansol ImRobert J Mitchell

Abstract

Violacein is a bisindole antibiotic that is effective against Gram-positive bacteria while the bacterial predator, Bdellovibrio bacteriovorus HD100, predates on Gram-negative strains. In this study, we evaluated the use of both together against multidrug resistant pathogens. The two antibacterial agents did not antagonize the activity of the other. For example, treatment of Staphylococcus aureus with violacein reduced its viability by more than 2,000-fold with or without B. bacteriovorus addition. Likewise, predation of Acinetobacter baumannii reduced the viability of this pathogen by more than 13,000-fold, regardless if violacein was present or not. When used individually against mixed bacterial cultures containing both Gram-positive and Gram-negative strains, violacein and B. bacteriovorus HD100 were effective against only their respective strains. The combined application of both violacein and B. bacteriovorus HD100, however, reduced the total pathogen numbers by as much as 84,500-fold. Their combined effectiveness was also demonstrated using a 4-species culture containing S. aureus, A. baumannii, Bacillus cereus and Klebsiella pneumoniae. When used alone, violacein and bacterial predation reduced the total population by onl...Continue Reading

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Citations

Dec 10, 2019·Environmental Microbiology·Seong Yeol ChoiRobert J Mitchell
Aug 14, 2019·Applied Microbiology and Biotechnology·Gayoung ChoRobert J Mitchell
Mar 17, 2020·Applied Microbiology and Biotechnology·Robert J MitchellSeong Yeol Choi
Oct 10, 2020·Computational and Structural Biotechnology Journal·Juana PérezJosé Muñoz-Dorado
Jan 20, 2021·Microbiology·Robert J Atterbury, Jess Tyson
Jan 21, 2021·Microorganisms·Nikita S LyakhovchenkoInna P Solyanikova
Mar 5, 2021·Bio-protocol·Emanuele Marine, Klaas M Pos
Mar 13, 2021·Journal of Biological Engineering·Seong Yeol ChoiRobert J Mitchell
Mar 18, 2021·Critical Reviews in Biotechnology·HyunA ParkKwon-Young Choi

Methods Mentioned

BETA
confocal microscopy

Related Concepts

Klebsiella pneumoniae
Antibiotics
Bacillus cereus
Bacterial Infections
Gram-Negative Bacteria
Gram-Positive Bacteria
Staphylococcus aureus
Anti-Bacterial Agents
Acinetobacter baumannii
Bdellovibrio bacteriovorus

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