Combined delivery of chondroitinase ABC and human induced pluripotent stem cell-derived neuroepithelial cells promote tissue repair in an animal model of spinal cord injury

Biomedical Materials
Tobias FührmannMolly S Shoichet

Abstract

The lack of tissue regeneration after traumatic spinal cord injury in animal models is largely attributed to the local inhibitory microenvironment. To overcome this inhibitory environment while promoting tissue regeneration, we investigated the combined delivery of chondroitinase ABC (chABC) with human induced pluripotent stem cell-derived neuroepithelial stem cells (NESCs). ChABC was delivered to the injured spinal cord at the site of injury by affinity release from a crosslinked methylcellulose (MC) hydrogel by injection into the intrathecal space. NESCs were distributed in a hydrogel comprised of hyaluronan and MC and injected into the spinal cord tissue both rostral and caudal to the site of injury. Cell transplantation led to reduced cavity formation, but did not improve motor function. While few surviving cells were found 2 weeks post injury, the majority of live cells were neurons, with only few astrocytes, oligodendrocytes, and progenitor cells. At 9 weeks post injury, there were more progenitor cells and a more even distribution of cell types compared to those at 2 weeks post injury, suggesting preferential survival and differentiation. Interestingly, animals that received cells and chABC had more neurons than animals ...Continue Reading

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Citations

Mar 9, 2019·Tissue Engineering. Part a·Marian H Hettiaratchi, Molly S Shoichet
Aug 28, 2019·Tissue Engineering. Part B, Reviews·Nureddin AshammakhiStephanie K Seidlits
Aug 10, 2018·Nature Communications·Letao YangKi-Bum Lee
Aug 10, 2019·Experimental Neurology·E MuirJ Fawcett
Jan 30, 2021·Advanced Functional Materials·Riya J MuckomDavid V Schaffer
Mar 23, 2021·Acta Biomaterialia·Sheng Yin, Yi Cao
Oct 17, 2020·Neural Regeneration Research·Lindsey H Forbes, Melissa R Andrews
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Jun 3, 2021·International Journal of Molecular Sciences·Pablo BonillaVictoria Moreno-Manzano
Dec 16, 2020·ACS Biomaterials Science & Engineering·Walaa A AbbasNageh K Allam
Aug 28, 2021·International Journal of Molecular Sciences·Kaneyasu Nishimura, Kazuyuki Takata
Nov 10, 2021·Biomacromolecules·Kelti A SmithMolly S Shoichet

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