Abstract
Donor leucocytes (DL) play an important role in rat liver transplant tolerance and their postoperative administration can convert rejection to tolerance. They appear to induce early activation, altered patterns of infiltration and death of recipient alloreactive T cells. The ability of immunosuppressive drugs to combine with DL administration was examined in a rat heart transplant model. Immediately after PVG to DA heterotopic heart transplantation, 6 x 10(7) spleen DL were injected. Cyclosporine A (CsA), 1.5 mg/kg/day, or methotrexate (MTX), 0.1 or 0.2 mg/kg/day, were given from day (d) 0 to d4 (early) or from d3 to d7 (delayed). Castanospermine (CAST) was administered from d0 to d7 at 100 or 300 mg/kg/day. In a separate experiment, transplanted hearts and recipient spleens were collected from treatment groups for analysis of infiltrate and cytokine mRNA expression. Delayed treatment with CsA or early treatment with MTX but not CAST combined with DL to result in prolonged graft survival. Recipients treated with DL and delayed CsA had a reduced level of intra-graft interleukin (IL)-2, interferon (IFN)-gamma, IL-4, and IL-4R mRNA expression and reduced infiltrate compared to DL alone. Early MTX plus DL led to almost complete inh...Continue Reading
References
Jan 1, 1991·Transplantation·W H BarberA G Diethelm
Nov 1, 1985·The Journal of Experimental Medicine·B M HallS E Dorsch
Mar 1, 1986·Kidney International·G A BishopD J Tiller
Jan 1, 1993·Transplant Immunology·G A BishopG W McCaughan
Aug 15, 1995·Transplantation·J SunG A Bishop
Jan 1, 1993·Transplant Immunology·T T PizarroL A Piccinini
May 30, 1996·Nature·C P LarsenT C Pearson
Dec 1, 1996·Transplant Immunology·P M GrochowiczD O Willenborg
Jan 15, 1997·Transplantation·C RicordiA G Tzakis
Feb 1, 1997·Transplantation Proceedings·S QianJ J Fung
Feb 1, 1997·Transplantation Proceedings·C RicordiA G Tzakis
Jun 13, 1998·Transplantation·A SharlandG A Bishop
Jul 17, 1998·The Journal of Clinical Investigation·L GenestierJ P Revillard
Oct 20, 1998·Transplant Immunology·C J CiesielskiL A Piccinini
Nov 21, 1998·Immunology·K L RokahrG A Bishop
Dec 29, 1998·Transplantation Proceedings·S SlavinT Prighozina
Dec 28, 1999·Transplantation·A SharlandG A Bishop
Feb 1, 2000·Transplantation·T Y TsuiH J Schlitt
Mar 23, 2001·Nature Immunology·O LantzJ P Di Santo
May 31, 2001·Immunology and Cell Biology·J L YinG A Bishop
Jul 27, 2001·Transplantation·C WangG A Bishop
Mar 1, 2002·Immunology and Cell Biology·G Alex BishopGeoffreyW McCaughan
Apr 20, 2002·Transplantation·Yiqun YanG Alex Bishop
May 1, 2002·Transplantation·Tung-Yu TsuiHans J Schlitt
Feb 18, 2003·Transplantation·Wen Hua HuangAnthony K House
Oct 28, 2003·Transplantation·Wen Hua HuangG Alex Bishop
Mar 10, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·H KaminskiP Merville
Citations
Jun 21, 2005·Transplantation Proceedings·T KanataniG A Bishop
Aug 20, 2010·Cell Transplantation·Jeng-Chang ChenRen-Huei Fu
Jan 21, 2011·Physiological Reviews·Tom L van BelleMatthias G von Herrath
Sep 20, 2020·Antiviral Research·Anil M TharappelHengli Tang