PMID: 11905516Mar 22, 2002Paper

Combined effect of oxygen and ammonia on the kinetics of ammonia elimination and oxygen consumption of adherent rat liver cells

The International Journal of Artificial Organs
G Catapano, Bartolo L De

Abstract

Oxygen is essential for the survival of isolated liver cells and its concentration is known to affect their viability and function. Recent reports have also shown that ammonia is eliminated at a rate depending on its concentration and that high ammonia concentrations may be cytotoxic to rat liver cells. Nonetheless, little quantitative information on the effect of either metabolite on liver cell reaction kinetics is available although important to the design of bioreactors for bioartificial livers (BALs). In this investigation, we characterized the dependence of the rate of oxygen consumption (OCR), ammonia elimination (AER) and urea synthesis (USR) on ammonia concentration at physiological (i.e., 43 and 72 mmHg) and supra-physiological (i.e., 134 mmHg) dissolved oxygen tensions. To this purpose, isolated rat liver cells were cultured in adhesion on collagen in a continuous-flow bioreactor optimised for the kinetic characterisation of liver cell metabolic reactions. Rates of the investigated reactions generally increased with increasing ammonia concentrations. OCR and USR significantly increased with increasing dissolved oxygen tensions, particularly at high ammonia concentrations. The actual dissolved oxygen tension significan...Continue Reading

References

Aug 10, 2000·Metabolic Engineering·U J BalisM Toner
Dec 5, 1992·Biotechnology and Bioengineering·A RotemM L Yarmush
Jan 20, 1993·Biotechnology and Bioengineering·S L NybergW S Hu
Aug 20, 1993·Biotechnology and Bioengineering·B D FoyM L Yarmush

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Citations

Dec 23, 2008·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Andrzej KinasiewiczAndrzej Werynski
Dec 17, 2010·World Journal of Gastrointestinal Surgery·Robert Afm Chamuleau
Feb 27, 2004·The International Journal of Artificial Organs·B CirilloG Catapano

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