Combined effect of synthetic protein, Mini-B, and cholesterol on a model lung surfactant mixture at the air-water interface

Biochimica Et Biophysica Acta
Aishik ChakrabortyPrajnaparamita Dhar

Abstract

The overall goal of this work is to study the combined effects of Mini-B, a 34 residue synthetic analog of the lung surfactant protein SP-B, and cholesterol, a neutral lipid, on a model binary lipid mixture containing dipalmitolphosphatidylcholine (DPPC) and palmitoyl-oleoyl-phosphatidylglycerol (POPG), that is often used to mimic the primary phospholipid composition of lung surfactants. Using surface pressure vs. mean molecular area isotherms, fluorescence imaging and analysis of lipid domain size distributions; we report on changes in the structure, function and stability of the model lipid-protein films in the presence and absence of varying composition of cholesterol. Our results indicate that at low cholesterol concentrations, Mini-B can prevent cholesterol's tendency to lower the line tension between lipid domain boundaries, while maintaining Mini-B's ability to cause reversible collapse resulting in the formation of surface associated reservoirs. Our results also show that lowering the line tension between domains can adversely impact monolayer folding mechanisms. We propose that small amounts of cholesterol and synthetic protein Mini-B can together achieve the seemingly opposing requirements of efficient LS: fluid enoug...Continue Reading

References

Dec 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S SchürchJ A Clements
Nov 1, 1988·Chemistry and Physics of Lipids·B D Fleming, K M Keough
Nov 14, 1997·Physiological Reviews·J R Wright
May 1, 1998·American Journal of Respiratory and Critical Care Medicine·J A Clements, M E Avery
Nov 14, 1998·Biochimica Et Biophysica Acta·J Goerke
Nov 14, 1998·Biochimica Et Biophysica Acta·R VeldhuizenF Possmayer
Feb 26, 2000·Molecular Medicine Today·B RobertsonT Curstedt
Nov 14, 2000·Physiological and Biochemical Zoology : PBZ·J R CoddS Orgeig
Dec 7, 2000·Biophysical Journal·E J VeldhuizenH P Haagsman
Oct 12, 2005·Biochimica Et Biophysica Acta·Lasantha GunasekaraMatthias Amrein
Dec 1, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·A J WaringJ A Zasadzinski
May 15, 2007·Chest·Timothy P Stevens, Robert A Sinkin
Jun 19, 2007·Neonatology·Frans J WaltherLarry M Gordon
Apr 9, 2008·Annual Review of Physical Chemistry·Ka Yee C Lee
Apr 25, 2008·Biochimica Et Biophysica Acta·Yi Y ZuoFred Possmayer
Jan 1, 2008·Soft Matter·Luka PocivavsekKa Yee C Lee
Mar 26, 2010·American Journal of Respiratory and Critical Care Medicine·Douglas F Willson, Neal J Thomas
May 21, 2010·Journal of the American Chemical Society·Michelle T DohmAnnelise E Barron
Jul 12, 2011·Critical Care Clinics·Krishnan RaghavendranR H Notter
Jan 10, 2012·Biophysical Journal·Prajnaparamita DharJoseph A Zasadzinski
Aug 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kyuhan KimJoseph A Zasadzinski
Jan 15, 2014·Biophysical Journal·Mridula V DwivediHans-Joachim Galla
Feb 15, 2014·Biochimica Et Biophysica Acta·Elena Lopez-Rodriguez, Jesús Pérez-Gil
Apr 16, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Aishik ChakrabortyPrajnaparamita Dhar

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Citations

Jul 2, 2020·Physical Chemistry Chemical Physics : PCCP·Sheikh I HossainSuvash C Saha
Apr 6, 2018·Journal of the Royal Society, Interface·Ian Williams, Todd M Squires
Sep 11, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Chris MirandaMichael R Morrow
Oct 14, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Laurianne Gravel-TattaAntonella Badia

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