Combined effect of testosterone and apocynin on nitric oxide and superoxide production in PMA-differentiated THP-1 cells

Biochimica Et Biophysica Acta
Packiasamy A R JulietLouis J Ignarro

Abstract

Human inducible nitric oxide synthase (iNOS) is most readily observed in macrophages from patients with inflammatory diseases like atherosclerosis. The aim of the present study was to find out the combined effect of male sex hormone; testosterone and apocynin (NADPH oxidase inhibitor) on cytokine-induced iNOS production. THP-1 cells were differentiated into macrophages by phorbol myristate acetate (PMA). Expression of iNOS was induced by the addition of cytokine mixture? Testosterone was added at different concentrations (10(-6)-10(-12) M) with apocynin (1 mM). Testosterone (10(-8), 10(-10) M) inhibited NOx production in cytokine-added THP-1 cells which was further confirmed by quantikine assay of iNOS protein and RT-PCR analysis. Testosterone treatment decreased 40% of superoxide anion production. Testosterone showed inhibition of NADPH oxidase, especially expression of p67phox and p47phox (cytosol subunits). Addition of testosterone with apocynin further decreased the expression of p67phox and p47phox subunits of NADPH oxidase. The findings of the present study suggest that, testosterone; the male androgen plays an important role in the prevention of atherogenesis. Even though apocynin does not have any role on NO production,...Continue Reading

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Citations

Jun 24, 2009·Prostate Cancer and Prostatic Diseases·J P LuJ H Pinthus
Jan 18, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Javad HabibiJames R Sowers
Jan 1, 2012·ISRN Inflammation·Ana Carolina de AlmeidaValdecir F Ximenes
Jan 23, 2008·The Journal of Sexual Medicine·Liming JinArthur L Burnett
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Mar 6, 2021·Nitric Oxide : Biology and Chemistry·Ya-Ying ChangTzu-Yu Lin
Mar 12, 2011·Journal of Agricultural and Food Chemistry·Marta RybickaDariusz Chlubek

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