Combined effects of fine particulate matter and lipopolysaccharide on apoptotic responses in NR8383 macrophages

Journal of Toxicology and Environmental Health. Part a
Qi XiongChaoying Li

Abstract

Alveolar macrophages (AM) are the predominant lung cells responsible for both ingestion and clearance of inhaled particulate matter (PM). The aims of this study were (1) to examine effects of fine PM on rat NR8383 cell line apoptosis, and (2) to determine whether NR8383 cell functions are further affected when exposed to fine PM in the presence of inflammation induced by lipopolysaccharide (LPS). Standard Reference Material 2786 (SRM 2786) for fine PM was used to measure the following parameters: cytotoxicity, apoptotic rate, Bax/Bcl-2 expression, nitric oxide (NO) production, and reactive oxygen species (ROS) generation in NR8383 cells. Data showed that SRM 2786 alone induced damage and apoptosis in NR8383 cells in a concentration-dependent manner as demonstrated by significant decrease in expression of Bcl-2 and increase in expression of Bax, suggesting fine PM might trigger apoptosis involving a mitochondria-mediated apoptotic pathway. In addition, there was elevated production of free radicals, such as NO and ROS, suggesting oxidative stress plays a role in the observed apoptotic responses. Further, LPS pretreatment enhanced apoptosis of NR8383 cells induced by SRM 2786. Consequently, data indicate that SRM 2786 triggered c...Continue Reading

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Citations

Apr 16, 2017·Journal of Neuroinflammation·Nicholas C WoodwardCaleb E Finch
Jun 20, 2020·Environmental Science and Pollution Research International·Mengyao WangZhijun Huang

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Methods Mentioned

BETA
Assay
fluorescence microscopy
enzyme-linked immunosorbent assay
flow cytometry

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