PMID: 10839474Jun 6, 2000Paper

Combined effects of okadaic acid and cadmium on lipid peroxidation and DNA bases modifications (m5dC and 8-(OH)-dG) in Caco-2 cells

Archives of Toxicology
A TraoreE E Creppy

Abstract

Okadaic acid (OA) is a marine toxin, a tumour promoter and an inducer of apoptosis. It mainly inhibits protein-phosphatases, protein synthesis and enhances lipid peroxidation. Cadmium (Cd) is known to be carcinogenic in animals and humans (group 1 according to the International Agency for Research on Cancer (IARC) classification). Cd also induces oxidative stress in living organisms. Since they are sometimes found simultaneously in mussels, we have evaluated in the present investigation, the lipid peroxidation, as malondialdehyde (MDA) production, in the variation of the ratios of 8-(OH)-dG/10(5)dG and m5dC/(dC + m5dC) induced by OA and/or Cd in Caco-2 cells. When cells were treated exclusively by OA (15 ng/ml) or Cd (0.625 and 5 microg/ml) for 24 h, protein synthesis was inhibited (by 42 +/- 5%, 18 +/- 13%, and 90 +/- 4% respectively) while MDA production was 2,235 +/- 129, 1710 +/- 20, and 11,496 +/-1,624 pmol/mg protein respectively. In addition, each toxicant induced modified bases in DNA; increases in oxidised bases and methylated dC. The combination of OA and cadmium was more cytotoxic and caused more DNA base modifications; the ratio m(5)dC/(m(5)dC + dC) was increased from 3 +/- 0.15 to 9 +/- 0.15 and the ratio 8-(OH)-dG...Continue Reading

Citations

May 27, 2005·The Science of the Total Environment·Lucie Ayi FanouHerman Autrup
Dec 31, 2002·Toxicology·Edmond Ekué CreppyMaria-Rosaria Carratú
Jun 29, 2011·Journal of Toxicology and Environmental Health. Part a·Fernanda Flórez-BarrósJuan Fernández-Tajes
Jul 11, 2006·International Journal of Environmental Research and Public Health·Pinto-Silva CarvalhoEdmond E Creppy
Aug 12, 2014·International Journal of Environmental Research and Public Health·Edmond E CreppyDaniel Cros
Jul 27, 2002·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Florence GeretMaria João Bebianno
Nov 30, 2005·Journal of Pineal Research·Gloria Benítez-King
Jun 23, 2016·Nutrition Reviews·Lieselot Y Hemeryck, Lynn Vanhaecke
Jul 11, 2006·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Isaac TúnezPedro Montilla
Jan 6, 2016·International Journal of Environmental Research and Public Health·Anthony SkipperPaul B Tchounwou
Mar 19, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daye Cheng, Hong Kong
Mar 29, 2003·Journal of Pineal Research·Isaac TúnezPedro Montilla López
Jan 14, 2020·Redox Biology·Marco GiorgioPier Giuseppe Pelicci

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