Abstract
Sphingomyelin (SM) is one of the major lipids in lipoproteins. However, its function in lipoprotein metabolism is unknown. In an attempt to understand the role that this lipid plays in modulation of lipoprotein lipase (LPL)-mediated hydrolysis, triolein-based emulsion particles containing 15% (physiological concentration) and 30% of the phospholipid content as SM together with phosphatidyl choline were used as substrate for the enzyme. Using a continuous fluorescence displacement assay to measure triglyceride (triolein) hydrolysis, it is shown that LPL activity was not modified by physiological concentrations of SM. However, under these assay conditions the presence of 30% SM inhibited LPL hydrolysis. SM and cholesterol (a normal component of the lipoprotein surface monolayer) become closely associated in phospholipid monolayers and bilayers. Incorporation of cholesterol into emulsion particles containing only PC increased LPL activity, but this increase was reduced by the additional presence of a physiological concentration (15%) of SM. These model studies suggest that the ratio, cholesterol:SM, in the monolayer may regulate the hydrolytic activity of the LPL. The production of ceramide by sphingomyelinase pre-treatment of emu...Continue Reading
References
May 12, 1975·Analytical Biochemistry·T G RedgraveC E West
Jan 1, 1976·Annual Review of Biophysics and Bioengineering·R Verger
Jun 5, 1992·Biochimica Et Biophysica Acta·T G RedgraveJ C Mamo
Jun 9, 1992·Biochimica Et Biophysica Acta·B CantinP Julien
Jan 1, 1992·Life Sciences·P VadasW Pruzanski
Aug 6, 1991·Biochemistry·C C KanR Bittman
Mar 12, 1991·Biochimica Et Biophysica Acta·M Koval, R E Pagano
Apr 23, 1991·European Journal of Biochemistry·C RojasG Bengtsson-Olivecrona
Jul 1, 1989·The Biochemical Journal·D C Wilton
Dec 13, 1982·Biochimica Et Biophysica Acta·R A DemelR L Jackson
May 6, 1994·Cell·R Kolesnick, D W Golde
Aug 2, 1996·The Journal of Biological Chemistry·S L SchisselI Tabas
Aug 9, 1996·The Journal of Biological Chemistry·D J Bolin, A Jonas
Dec 1, 1995·Atherosclerosis·M MenschikowskiW Jaross
Sep 15, 1996·The Journal of Clinical Investigation·S L SchisselI Tabas
Jul 1, 1996·Atherosclerosis·D J Betteridge
Sep 27, 1996·Biochimica Et Biophysica Acta·R OthmanD C Wilton
Oct 1, 1996·Current Opinion in Lipidology·U Beisiegel, R W St Clair
Feb 1, 1997·The Biochemical Journal·L I Lobo, D C Wilton
Feb 18, 1997·Biochimica Et Biophysica Acta·M JungnerJ P Slotte
Citations
Sep 23, 2000·Chemistry and Physics of Lipids·J P Slotte
Jan 29, 2003·Life Sciences·Rocio AbiaFrancisco J G Muriana
Nov 18, 2000·Biochimica Et Biophysica Acta·A G Buckland, D C Wilton
Dec 8, 2009·Journal of Lipid Research·Hiroyuki NakamuraToshihiko Murayama
Mar 1, 2012·Biochimica Et Biophysica Acta·Papasani Venkata SubbaiahCatherine A Reardon
May 18, 2011·Chemistry and Physics of Lipids·Yi-Ling ChiouLong-Sen Chang
May 21, 2013·Progress in Lipid Research·J Peter Slotte
Jul 20, 2001·Medical Hypotheses·P V Subbaiah, R M Sargis
Dec 14, 2004·Chemistry and Physics of Lipids·Jocelyne PiretMarie-Paule Mingeot-Leclercq
Dec 7, 2006·Journal of Lipid Research·Dev K Singh, Papasani V Subbaiah
Oct 28, 2005·Journal of Lipid Research·Ake Nilsson, Rui-Dong Duan
Sep 16, 2005·Journal of Lipid Research·Papasani V SubbaiahKewei Wang
Jan 5, 2016·Biological & Pharmaceutical Bulletin·Shin-ya Morita
Dec 14, 1999·The Journal of Biological Chemistry·P V SubbaiahK Wang
Jul 21, 2000·Biochimica Et Biophysica Acta·H SaitoT Handa
Sep 23, 2003·Cellular Signalling·Nehmann Al-MakdissyMireille Donner