PMID: 9421185Jan 8, 1998Paper

Combined effects of sphingomyelin and cholesterol on the hydrolysis of emulsion particle triolein by lipoprotein lipase

Biochimica Et Biophysica Acta
L I Lobo, D C Wilton

Abstract

Sphingomyelin (SM) is one of the major lipids in lipoproteins. However, its function in lipoprotein metabolism is unknown. In an attempt to understand the role that this lipid plays in modulation of lipoprotein lipase (LPL)-mediated hydrolysis, triolein-based emulsion particles containing 15% (physiological concentration) and 30% of the phospholipid content as SM together with phosphatidyl choline were used as substrate for the enzyme. Using a continuous fluorescence displacement assay to measure triglyceride (triolein) hydrolysis, it is shown that LPL activity was not modified by physiological concentrations of SM. However, under these assay conditions the presence of 30% SM inhibited LPL hydrolysis. SM and cholesterol (a normal component of the lipoprotein surface monolayer) become closely associated in phospholipid monolayers and bilayers. Incorporation of cholesterol into emulsion particles containing only PC increased LPL activity, but this increase was reduced by the additional presence of a physiological concentration (15%) of SM. These model studies suggest that the ratio, cholesterol:SM, in the monolayer may regulate the hydrolytic activity of the LPL. The production of ceramide by sphingomyelinase pre-treatment of emu...Continue Reading

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Citations

Sep 23, 2000·Chemistry and Physics of Lipids·J P Slotte
Nov 18, 2000·Biochimica Et Biophysica Acta·A G Buckland, D C Wilton
May 18, 2011·Chemistry and Physics of Lipids·Yi-Ling ChiouLong-Sen Chang
May 21, 2013·Progress in Lipid Research·J Peter Slotte
Jul 20, 2001·Medical Hypotheses·P V Subbaiah, R M Sargis
Dec 14, 2004·Chemistry and Physics of Lipids·Jocelyne PiretMarie-Paule Mingeot-Leclercq
Oct 28, 2005·Journal of Lipid Research·Ake Nilsson, Rui-Dong Duan
Sep 16, 2005·Journal of Lipid Research·Papasani V SubbaiahKewei Wang

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