Combined fine mapping, genetic diversity, and transcriptome profiling reveals that the auxin transporter gene ns plays an important role in cucumber fruit spine development

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Qing XieSheng-Ping Zhang

Abstract

Map-based cloning was used to identify the ns gene, which was involved in the formation of cucumber numerous fruit spines together with other genes under regulation by plant hormone signal transduction. The cucumber (Cucumis sativus) fruit spine density has an important impact on the commercial value. However, little is known about the regulatory mechanism for the fruit spine formation. Here, we identified NUMEROUS SPINES (NS), which regulate fruit spine development by modulating the Auxin signaling pathway. We fine-mapped the ns using a 2513 F2 population derived from NCG122 (numerous fruit spines line) and NCG121 (few fruit spines line), and showed that NS encoded auxin transporter-like protein 3. Genetic diversity analysis of the NS gene in natural populations revealed that one SNP and one InDel in the coding region of ns are co-segregated with the fruit spine density. The NS protein sequence was highly conserved among plants, but its regulation of fruit spine development in cucumber seems to be a novel function. Transcriptome profiling indicated that the plant hormone signal transduction-related genes were highly enriched in the up-regulated genes in NCG122 versus NCG121. Moreover, expression pattern analysis of the auxin s...Continue Reading

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Citations

Sep 23, 2018·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kailiang BoXingfang Gu
Feb 13, 2021·The Plant Journal : for Cell and Molecular Biology·Yaqi ZhangXingwang Liu
Jun 29, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Kiros GebretsadikKailiang Bo

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Datasets Mentioned

BETA
SSR04219

Methods Mentioned

BETA
reverse transcription PCR
PCR
RNA-Seq

Software Mentioned

Map
Primer
DESeq
in
DGE
Perl scripts
TopHat
MEGA
Join
SMART

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