PMID: 489419Aug 1, 1979Paper

Combined histochemical and biochemical investigation to the reliability of the demonstration of arylsulphatase activity in cryostat sections

Histochemistry
C M van der LoosA C Jöbsis

Abstract

The reliability of the enzyme histochemical technique, for the demonstration of arylsulphatase activity, using 6-bromo-2-naphthylsulphate as a substrate, is biochemically tested by using partly purified lysosome and microsome preparations from fresh human placenta tissue. Microsomes from frozen placenta with an arylsulphatase deficiency and lysosomes from rat liver, are also investigated. For the biochemical test methods, 6-bromo-2-naphthylsulphate and p-nitrocatecholsulphate are used as substrates. Under similar reaction conditions, varying the pH of the incubation medium and adding inhibitors or activators, the histochemical and biochemical reactions are compared. The results of this study show that the enzyme histochemical technique--except for some limitations--is suitable for the demonstration of microsomal arylsulphatase in cryostat sections.

References

Oct 7, 1978·Lancet·L J ShapiroR S Wells
Oct 1, 1978·Archives of Disease in Childhood·G KoppeA C Jöbsis
Nov 1, 1975·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J Koudstaal
Jan 1, 1973·The Journal of Clinical Endocrinology and Metabolism·J T FranceG C Liggins
Jun 1, 1972·The Biochemical Journal·J O DollyF A Rose
Feb 1, 1954·The Biochemical Journal·K S DODGSONJ THOMAS
Sep 1, 1955·Canadian Journal of Biochemistry and Physiology·R VIALA, R GIANETTO
Aug 1, 1957·The Biochemical Journal·A B ROY
Mar 1, 1958·The Biochemical Journal·A B ROY
Apr 1, 1964·Clinica Chimica Acta; International Journal of Clinical Chemistry·J GNIOT, L M DZIALOSZYNSKI
Jan 1, 1955·The Biochemical Journal·K S DODGSONJ THOMAS

❮ Previous
Next ❯

Citations

Jan 1, 1985·Archives of Dermatological Research·O ProstG L Adessi
Jan 1, 1982·Journal of Inherited Metabolic Disease·F A Rose
Jan 1, 1984·Journal of Inherited Metabolic Disease·A Marinkovic-Ilsen, M L Williams
Dec 1, 1983·Journal of Steroid Biochemistry·C M van der LoosA C Jöbsis
Dec 1, 1980·European Journal of Obstetrics, Gynecology, and Reproductive Biology·A P StruykP E Treffers
Jan 1, 1984·Journal of Inherited Metabolic Disease·C M van der LoosA C Jöbsis
Jan 1, 1985·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·S Partanen
May 1, 1983·The British Journal of Dermatology·A C JöbsisC M Van der Loos
Jul 1, 1980·The British Journal of Dermatology·W P de GrootH W de Bruijn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.