Combined influence of basal media and fibroblast growth factor on the expansion and differentiation capabilities of adipose-derived stem cells

Cell Regeneration
Mark AhearneAmy P Lynch

Abstract

Interest in adipose-derived stem cells (ASCs) has increased in recent years due to their multi-linage differentiation capabilities. While much work has been done to optimize the differentiation media, few studies have focused on examining the influence of different expansion media on cell behavior. In this study, three basal media (low glucose Dulbecco's modified Eagle's medium (DMEM), high glucose DMEM and DMEM-F12) supplemented with or without fibroblast growth factor 2 (FGF) were examined to assess their suitability for expanding ASCs. Flow cytometry, colony-forming unit assays (CFU-Fs) and differentiation assays were utilized to study cell behavior. High glucose media CFU-Fs produced fewest colonies while the addition of FGF increased colony size. By passage 2, the majority of cells were positive for CD44, 45, 73, 90 and 105 and negative for CD14, 31 and 45, indicating a mesenchymal phenotype. A sub-population of CD34 positive cells was present among passage 2 cells; however, by passage 4 the cells were negative for CD34. FGF has a negative effective on passage 4 ASC adipogenesis and high glucose media plus FGF-enhanced osteogenic capacity of passage 4 ASCs. FGF supplemented basal media were most suitable for chondrogenesis...Continue Reading

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Citations

Oct 20, 2015·Journal of the Mechanical Behavior of Biomedical Materials·Mark Ahearne, Aron Coyle
Apr 14, 2016·Annals of Plastic Surgery·Vipra GunetaCleo Choong
Nov 2, 2016·Frontiers in Cell and Developmental Biology·Carla DesselsMichael S Pepper
Jul 17, 2019·Investigative Ophthalmology & Visual Science·Aurelie Dos SantosSophie X Deng
Apr 23, 2020·Scientific Reports·Promita BhattacharjeeMark Ahearne

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Methods Mentioned

BETA
PCR

Software Mentioned

Flowing
ImageJ

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