Combined intraoperative administration of a histone deacetylase inhibitor and a neurokinin-1 receptor antagonist synergistically reduces intra-abdominal adhesion formation in a rat model

Surgery
Michael R CassidyA F Stucchi

Abstract

Intra-abdominal adhesions are the most frequent postoperative complication after abdominopelvic surgery. Our laboratory has previously shown that an intraoperative peritoneal lavage containing either the histone deacetylase inhibitor valproic acid (VPA) or a neurokinin-1 receptor antagonist (NK-1RA) reduced adhesions by approximately 50% in a rat model. The objective of this study was to determine whether the combination of these 2 drugs was more effective in reducing adhesions than either alone. Rats underwent laparotomy with creation of peritoneal ischemic buttons to induce adhesions. A single dose of VPA (25 mg/kg), NK-1RA (50 mg/kg), a combination of both, or 0.9% saline was lavaged intraperitoneally just before wound closure. On postoperative day 7, adhesions were quantified. To investigate early mechanisms of adhesiogenesis, adhesions were created as described and adhesive button tissue was harvested at 30 minutes and 3 hours postoperatively and fibrinogen and vascular endothelial growth factor (VEGF) protein levels, both indices of peritoneal extravasations, were determined by Western blot analysis. Peritoneal fluid was collected in similar experiments at 30 minutes, and 3 and 6 hours to measure fibrinolytic activity, an...Continue Reading

References

Nov 1, 1975·British Medical Journal·A Richens, S Ahmad
Aug 6, 2000·Fertility and Sterility·B W HellebrekersT Kooistra
Dec 4, 2003·Expert Opinion on Pharmacotherapy·Lisa Patel, Celeste Lindley
Jun 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Karen L ReedJames M Becker
Jan 18, 2005·Fertility and Sterility·Bart W J HellebrekersTrudy C M Trimbos-Kemper
Jun 15, 2007·The Journal of Surgical Research·Tatiana SeguraJeffrey A Hubbell
Jul 14, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Karen L ReedJames M Becker
Oct 27, 2007·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·J B C van der Wal, J Jeekel
Oct 27, 2007·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·H van Goor
Oct 27, 2007·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·D Stanciu, D Menzies
Oct 27, 2007·Colorectal Disease : the Official Journal of the Association of Coloproctology of Great Britain and Ireland·M C ParkerH Ellis
Aug 19, 2008·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Rizal LimJames M Becker
Dec 29, 2009·The Journal of Surgical Research·Brian C Ward, Alyssa Panitch
Aug 31, 2011·The British Journal of Surgery·B W J Hellebrekers, T Kooistra
Dec 8, 2011·World Journal of Gastroenterology : WJG·Willy ArungOlivier Detry
Dec 16, 2011·World Journal of Gastroenterology : WJG·Hussein M Atta
Feb 3, 2012·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Christoph BrochhausenC James Kirkpatrick
May 10, 2012·Archives of Gynecology and Obstetrics·Markus WallwienerAndreas D Hartkopf
May 11, 2012·Journal of Materials Science. Materials in Medicine·Christoph BrochhausenC James Kirkpatrick
Oct 5, 2013·BMJ : British Medical Journal·Richard P G ten BroekHarry van Goor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.