Abstract
Adipose tissue-derived stromal cells (ASCs) are increasingly being studied for their usefulness in regenerative medicine. However, limited life span and donor-dependent variation of primary cells such as ASCs present major hurdles to controlled and reproducible experiments. We therefore aimed to establish immortalized ASC cell lines that provide steady supply of homogeneous cells for in vitro work while retain essential features of primary cells. To this end, combinations of human telomerase reverse transcriptase (hTERT), murine Bmi-1, and SV40 large T antigen (SV40T) were introduced by lentiviral transduction into ASCs. The resulting cell lines ASC(hTERT), ASC(Bmi-1), ASC(Bmi-1+hTERT) and ASC(SV40T+hTERT) were tested for transgene expression, telomerase activity, surface immunomarkers, proliferation, osteogenic and adipogenic differentiation, karyotype, tumorigenicity, and cellular senescence. All cell lines have maintained expression of characteristic surface immunomarkers, and none was tumorigenic. However, ASC(Bmi-1) had limited replicative potential, while the rapidly proliferating ASC(SV40T+hTERT) acquired chromosomal aberrations, departed from MSC phenotype, and lost differentiation capacity. ASC(hTERT) and ASC(hTERT+Bmi...Continue Reading
References
Jun 24, 1998·Genes & Development·J WangG J Hannon
Nov 26, 1998·Experimental Cell Research·K K JhaH L Ozer
Oct 28, 1999·Journal of Cellular Biochemistry·D G PhinneyD J Prockop
Jun 4, 2002·Nature Biotechnology·Janne L SimonsenMoustapha Kassem
Oct 28, 2003·Human Gene Therapy·Christophe Cudré-MaurouxDidier Trono
Jan 15, 2004·The Journal of Clinical Investigation·In-Kyung ParkMichael F Clarke
Apr 27, 2004·Oncogene·Nedime SerakinciMoustapha Kassem
Jan 14, 2005·Molecular Biology of the Cell·Masanori TeraiTohru Kiyono
Jun 1, 2005·Molecular and Cellular Biology·Taisuke MoriAkihiro Umezawa
Nov 11, 2006·Biochemical and Biophysical Research Communications·Xiaohong ZhangTsuneo A Takahashi
May 1, 2007·Regenerative Medicine·Nedime SerakinciW Nicol Keith
May 1, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ramakrishnaiah SiddappaJan de Boer
Apr 1, 2008·Transplantation Proceedings·L L WeiY R Lu
Apr 1, 2008·Transplantation Proceedings·K GaoJ Q Cheng
Jul 12, 2008·Cell Proliferation·G P HuangJ F Wang
Jun 23, 2010·Human Gene Therapy·Pierre Charbord
Dec 28, 2010·Advanced Drug Delivery Reviews·Wilhelm K AicherGerd Klein
Feb 10, 2011·Stem Cells·Benjamin Levi, Michael T Longaker
Apr 26, 2011·Journal of Translational Medicine·Tian Sheng ChenSai Kiang Lim
May 17, 2011·International Journal of Oncology·Emi YamaokaKeiko Hiyama
Citations
Jun 3, 2014·Stem Cell Research & Therapy·Luigi BalducciGiulio Alessandri
Dec 4, 2015·Biomolecules·Xiaozeng LinDamu Tang
Jul 29, 2015·Prion·WooJae ChoiGoo Jang
Mar 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Deep KwatraShrikant Anant
Aug 8, 2014·Stem Cells and Development·Áron SzepesiKatalin Német
Nov 2, 2014·PloS One·Magdalena KoszarskaGergely Szakacs
Sep 17, 2014·Scientific Reports·S RinaldiC Ventura
Apr 13, 2018·Infectious Agents and Cancer·Silvia GiuncoAnita De Rossi
Aug 17, 2018·Experimental Biology and Medicine·Dóra MihályZoltán Sápi
Feb 10, 2018·Scientific Reports·Izabel PatikCsilla Özvegy-Laczka
Feb 11, 2019·Biotechnology and Bioengineering·Janina BurkCornelia Kasper
Jun 4, 2014·International Journal of Oncology·Yong QinShi-Ming Yang
Apr 20, 2017·Stem Cell Research & Therapy·Zvenyslava Husak, Michael N Dworzak
Dec 13, 2017·Journal of Cellular Biochemistry·Jia FangJinlian Hua
Aug 8, 2018·International Journal of Molecular Medicine·Shu-Yu FangYang Bi
Apr 7, 2020·Cell Transplantation·Jia-Jie TanXiang-Ping Li
Jan 11, 2019·Cell & Bioscience·Yiming WangMing Pei
Dec 22, 2016·Stem Cells International·Áron SzepesiKatalin Német
Oct 18, 2018·Diagnostic Pathology·Dóra MihályZoltán Sápi
Dec 19, 2019·International Journal of Molecular Sciences·M Piñeiro-RamilS M Díaz-Prado
Mar 6, 2019·Nature Genetics·Alexander RauchSusanne Mandrup
Nov 11, 2020·Nature Genetics·Alessandra FascianiAlessio Zippo
Apr 11, 2021·Scientific Reports·Azadeh HaghighitalabAhmad Reza Bahrami