Combined Labelled and Label-free SERS Probes for Triplex Three-dimensional Cellular Imaging

Scientific Reports
Yong ChenJiming Hu

Abstract

Cells are complex chemical systems, where the molecular composition at different cellular locations and specific intracellular chemical interactions determine the biological function. An in-situ nondestructive characterization of the complicated chemical processes (like e.g. apoptosis) is the goal of our study. Here, we present the results of simultaneous and three-dimensional imaging of double organelles (nucleus and membrane) in single HeLa cells by means of either labelled or label-free surface-enhanced Raman spectroscopy (SERS). This combination of imaging with and without labels is not possible when using fluorescence microscopy. The SERS technique is used for a stereoscopic description of the intrinsic chemical nature of nuclei and the precise localization of folate (FA) and luteinizing hormone-releasing hormone (LHRH) on the membrane under highly confocal conditions. We also report on the time-dependent changes of cell nuclei as well as membrane receptor proteins during apoptosis analyzed by statistical multivariate methods. The multiplex three-dimensional SERS imaging technique allows for both temporal (real time) and spatial (multiple organelles and molecules in three-dimensional space) live-cell imaging and therefore ...Continue Reading

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Citations

Apr 12, 2017·Nanomaterials·Gamze Kuku, Mustafa Culha
Jul 11, 2019·Analytical and Bioanalytical Chemistry·Yanting ShenShuping Xu
Mar 17, 2017·Annual Review of Analytical Chemistry·Lauren E JamiesonDuncan Graham
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Feb 6, 2020·Proceedings of the National Academy of Sciences of the United States of America·Wei ZhangAnhong Zhou
Jan 2, 2021·Colloids and Surfaces. B, Biointerfaces·Ping TangLiyun Zhong
Sep 19, 2017·ACS Applied Materials & Interfaces·Renyong LiuZhongping Zhang
Oct 20, 2018·Analytical Chemistry·Chamari S WijesooriyaEmily A Smith
Aug 23, 2018·ACS Applied Materials & Interfaces·Victoria M SzlagChristy L Haynes

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Methods Mentioned

BETA
surface-enhanced Raman scattering
surface plasmon resonance
flow cytometry
confocal microscopy

Software Mentioned

MATLAB

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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